Comparative genomics of Staphylococcus aureus associated with subclinical and clinical bovine mastitis

PloS One
Lis S RochaAndréa de O B Ribon

Abstract

Many efforts have been made to understand the pathogenesis of bovine mastitis to reduce losses and promote animal welfare. Staphylococcus aureus may cause bovine clinical mastitis, but it is mainly associated with subclinical infection, which is usually persistent and can easily reoccur. Here, we conducted a comparative genomic analysis between strains of S. aureus causing subclinical infection (Sau170, 302, 1269, 1364), previously sequenced by our group, and two well-characterized strains causing clinical mastitis (N305 and RF122) to find differences that could be linked to mastitis outcome. A total of 146 virulence-associated genes were compared and no appreciable differences were found between the bacteria. However, several nonsynonymous single nucleotide polymorphisms (SNPs) were identified in genes present in the subclinical strains when compared to RF122 and N305, especially in genes encoding host immune evasion and surface proteins. The secreted and surface proteins predicted by in silico tools were compared through multidimensional scaling analysis (MDS), revealing a high degree of similarity among the strains. The comparison of orthologous genes by OrthoMCL identified a membrane transporter and a lipoprotein as exclusi...Continue Reading

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Methods Mentioned

BETA
MDS
PCR
electrophoresis
PCRs

Software Mentioned

ggplot2
Clustal
LipoP
Bowtie2
PSORTb
Basic Variant Detection
R
Clustal Omega
Sequence Demarcation Tool
BUSCO

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