Comparative genomics profiling of clinical isolates of Aeromonas salmonicida using DNA microarrays

BMC Genomics
John H E NashLaura L Brown

Abstract

Aeromonas salmonicida has been isolated from numerous fish species and shows wide variation in virulence and pathogenicity. As part of a larger research program to identify virulence genes and candidates for vaccine development, a DNA microarray was constructed using a subset of 2024 genes from the draft genome sequence of A. salmonicida subsp. salmonicida strain A449. The microarray included genes encoding known virulence-associated factors in A. salmonicida and homologs of virulence genes of other pathogens. We used microarray-based comparative genomic hybridizations (M-CGH) to compare selected A. salmonicida sub-species and other Aeromonas species from different hosts and geographic locations. Results showed variable carriage of virulence-associated genes and generally increased variation in gene content across sub-species and species boundaries. The greatest variation was observed among genes associated with plasmids and transposons. There was little correlation between geographic region and degree of variation for all isolates tested. We have used the M-CGH technique to identify subsets of conserved genes from amongst this set of A. salmonicida virulence genes for further investigation as potential vaccine candidates. Unli...Continue Reading

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Jan 13, 2010·Clinical Microbiology Reviews·J Michael Janda, Sharon L Abbott
Sep 19, 2006·Journal of Bacteriology·Rekha SeshadriJohn F Heidelberg
Mar 14, 2007·Journal of Bacteriology·Amanda L LloydHarry L T Mobley
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Methods Mentioned

BETA
Electrophoresis
PCR
chip

Software Mentioned

BASE
MEV
ArrayPro Analyzer
BASE BioArray Software Environment
Glimmer
Primer3
BLAST
BLASTP
TIGR

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