Comparative Genomics Reveal a Flagellar System, a Type VI Secretion System and Plant Growth-Promoting Gene Clusters Unique to the Endophytic Bacterium Kosakonia radicincitans

Frontiers in Microbiology
Matthias BeckerSilke Ruppel

Abstract

The recent worldwide discovery of plant growth-promoting (PGP) Kosakonia radicincitans in a large variety of crop plants suggests that this species confers significant influence on plants, both in terms of yield increase and product quality improvement. We provide a comparative genome analysis which helps to unravel the genetic basis for K. radicincitans' motility, competitiveness and plant growth-promoting capacities. We discovered that K. radicincitans carries multiple copies of complex gene clusters, among them two flagellar systems and three type VI secretion systems (T6SSs). We speculate that host invasion may be facilitated by different flagella, and bacterial competitor suppression by effector proteins ejected via T6SSs. We found a large plasmid in K. radicincitans DSM 16656T, the species type strain, that confers the potential to exploit plant-derived carbon sources. We propose that multiple copies of complex gene clusters in K. radicincitans are metabolically expensive but provide competitive advantage over other bacterial strains in nutrient-rich environments. The comparison of the DSM 16656T genome to genomes of other genera of enteric plant growth-promoting bacteria (PGPB) exhibits traits unique to DSM 16656T and K....Continue Reading

References

Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·G FelixT Boller
Apr 1, 2000·Applied and Environmental Microbiology·J A KlappenbachT M Schmidt
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·E DíazJ L García
Jul 27, 2002·Annual Review of Microbiology·John S Mattick
Jun 2, 2004·Journal of Molecular Microbiology and Biotechnology·Linda L McCarter
Jul 21, 2004·Nature Reviews. Microbiology·Ray Dixon, Daniel Kahn
Feb 3, 2005·Journal of Bacteriology·Linda L McCarter
May 20, 2005·Systematic and Applied Microbiology·Peter KämpferRainer Remus
Jul 25, 2006·Archives of Microbiology·En Tao WangEsperanza Martínez-Romero
Feb 22, 2008·Current Opinion in Microbiology·Lewis Eh BingleMark J Pallen
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
May 21, 2010·PLoS Genetics·Safiyh TaghaviSébastien Monchy
Mar 3, 2011·Molecular Plant-microbe Interactions : MPMI·Angela R Records
Jun 16, 2011·Nucleic Acids Research·You ZhouDavid S Wishart
Dec 20, 2011·Standards in Genomic Sciences·Kristen M DeangelisTerry C Hazen
Sep 12, 2012·Journal of Bacteriology·Katja WitzelSilke Ruppel
Sep 27, 2013·PloS One·Wing-Yee LiuFrederick Chi-Ching Leung
Dec 18, 2013·Cell Host & Microbe·Brian T HoJohn J Mekalanos
Mar 20, 2014·Bioinformatics·Torsten Seemann
May 2, 2014·Frontiers in Plant Science·Anton HartmannPeter Schröder
Jul 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sebastian BubendorferKai M Thormann
Aug 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jianguo YangYi-Ping Wang
Mar 18, 2015·FEMS Microbiology Ecology·Alexandra B WolfGeorge A Kowalchuk
Jun 17, 2015·Trends in Microbiology·Ute Römling, Michael Y Galperin
Nov 10, 2015·Trends in Microbiology·Francesca R CianfanelliSarah J Coulthurst
Apr 17, 2016·Applied and Environmental Microbiology·Florence MusJohn W Peters
May 27, 2016·Microbiology Spectrum·Christopher J Alteri, Harry L T Mobley
Oct 27, 2016·Nature Microbiology·Benjamin R K RollerThomas M Schmidt
Feb 23, 2017·Frontiers in Microbiology·Hafiz A S TahirXuewen Gao

❮ Previous
Next ❯

Citations

Oct 15, 2019·Molecular Plant-microbe Interactions : MPMI·Susan MosquitoVittorio Venturi
Dec 14, 2019·World Journal of Microbiology & Biotechnology·Mauricio Cruz BarreraAnant V Patel
Apr 26, 2020·Applied and Environmental Microbiology·Susan MosquitoVittorio Venturi
Apr 24, 2019·International Journal of Molecular Sciences·Artur PinskiRobert Hasterok
Nov 27, 2020·Microorganisms·Gylaine Vanissa Tchuisseu TchakountéSilke Ruppel
Jan 5, 2021·World Journal of Microbiology & Biotechnology·Cecilia TauléFederico Battistoni

❮ Previous
Next ❯

Datasets Mentioned

BETA
CP018016.1
CP018017.1
CP018018.1

Methods Mentioned

BETA
electrophoresis
PCR
transmission electron microscopy
ICE
Illumina sequencing

Software Mentioned

RS
SMRT Portal
BLASTp
MAUVE
MUSCLE
PHAST
PhyCA
[UNK] Analysis
Rapid Annotations using Subsystems Technology ( RAST )
Interpro

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.