Comparative genotyping and phenotyping of Aspergillus fumigatus isolates from humans, dogs and the environment

BMC Microbiology
Ivan D ValdesHans de Cock

Abstract

Aspergillus fumigatus is a ubiquitous saprotrophic fungus and an opportunistic pathogen of humans and animals. Humans and animals can inhale hundreds of A. fumigatus spores daily. Normally this is harmless for humans, but in case of immunodeficiency, invasive pulmonary aspergillosis (IPA) can develop with a high mortality rate. A. fumigatus also causes non-invasive mycoses like sino-nasal aspergillosis (SNA) in dogs. In this study we compared A. fumigatus isolates from humans with suspected IPA, dogs with SNA, and a set of environmental isolates. Phylogenetic inference based on calmodulin (CaM) and beta-tubulin (benA) sequences did not reveal A. fumigatus sub-groups linked to the origin of the isolates. Genotyping and microsatellite analysis showed that each dog was infected by one A. fumigatus genotype, whereas human patients had mixed infections. Azole resistance was determined by antifungal susceptibility testing and sequencing of the cyp51A gene. A total of 12 out of 29 human isolates and 1 out of 27 environmental isolates were azole resistant. Of the azole resistant strains, 11 human isolates showed TR34/L98H (n = 6) or TR46/Y121F/T289A (n = 5). Phenotypically, isolates from dogs were more variable in growth speed and morp...Continue Reading

References

May 1, 1976·Clinical Allergy·J MullinsA Seaton
Apr 1, 1995·Applied and Environmental Microbiology·N L Glass, G C Donaldson
Apr 9, 1999·Clinical Microbiology Reviews·J P Latgé
Jul 14, 1999·Diagnostic Microbiology and Infectious Disease·M F VandenBerghA Voss
Feb 27, 2003·Antimicrobial Agents and Chemotherapy·T M Diaz-GuerraJ L Rodriguez-Tudela
May 17, 2005·Journal of Comparative Pathology·D PeetersC Clercx
Sep 24, 2005·Medical Mycology·Derek PaisleyDavid W Denning
Aug 19, 2007·The Veterinary Clinics of North America. Small Animal Practice·Dominique Peeters, Cécile Clercx
Apr 12, 2008·PLoS Genetics·Natalie D FedorovaWilliam C Nierman
Jul 9, 2008·Medical Mycology·Michael J Day
Sep 30, 2008·The Journal of Antimicrobial Chemotherapy·Eilidh MowatGordon Ramage
Oct 14, 2009·Clinical Microbiology Reviews·Stacy J Park, Borna Mehrad
Jul 14, 2012·The Journal of Small Animal Practice·M J Sharman, C S Mansfield
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
May 15, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jan W M van der LindenPaul E Verweij
Dec 19, 2013·PLoS Pathogens·Kyung J Kwon-Chung, Janyce A Sugui
Dec 11, 2014·Studies in Mycology·R A SamsonJ C Frisvad
Jul 15, 2015·The Journal of Antimicrobial Chemotherapy·J FuhrenJ G Kusters
Oct 22, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Paul E VerweijJacques F Meis
Dec 10, 2015·Mycoses·Pieter P A LestradePaul E Verweij
Feb 28, 2016·Molecular Biology and Evolution·Jaime Huerta-CepasPeer Bork
Apr 20, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R H JensenM C Arendrup
Oct 30, 2016·The Lancet Infectious Diseases·Paul E VerweijWillem J G Melchers
Jan 13, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jacques F MeisPaul E Verweij
Sep 16, 2017·The Journal of Infectious Diseases·Anuradha ChowdharyJacques F Meis
Feb 3, 2018·Environmental Microbiology·Anuradha Chowdhary, Jacques F Meis
Feb 27, 2018·Fungal Genetics and Biology : FG & B·Eloise BallardAdilia Warris

❮ Previous
Next ❯

Citations

Dec 5, 2019·BMJ Open Ophthalmology·Katie KerrAmy Jayne McKnight
Apr 3, 2020·Medical Mycology·Martin van EijkHans de Cock
Oct 14, 2020·Environmental Microbiology·Ashley V NyweningJarrod R Fortwendel

❮ Previous
Next ❯

Methods Mentioned

BETA
PCA
PCR
gel filtration
genotyping
biopsies
bronchoalveolar lavage

Software Mentioned

R package poppr
Figtree
ETE Toolkit
ImageJ

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.