Comparative high-performance liquid chromatography enantioseparations on polysaccharide based chiral stationary phases prepared by coating totally porous and core-shell silica particles

Journal of Chromatography. a
Ketevan LomsadzeBezhan Chankvetadze

Abstract

This article reports comparative high-performance liquid chromatographic separations of enantiomers with chiral stationary phases (CSPs) prepared by coating cellulose tris(4-chloro-3-methylphenylcarbamate) on totally porous and on core-shell type silica of comparable particle diameter. Several interesting observations were made: (1) the selectivity of separation was higher on core-shell type CSP compared to totally porous CSP at comparable content of chiral selector (polysaccharide derivative); (2) much flatter dependence of plate height on the mobile phase flow rate was observed for columns packet with CSP prepared with core-shell silica compared to the ones packed with CSPs prepared with totally porous particles; (3) at low mobile phase flow rates core-shell CSP provided lower resolving ability compared to a commercially available CSP having four times higher content of chiral selector along with higher retention of chiral analytes. However, at high flow rates core-shell type CSP performed similarly or better than the commercial column in regards of plate count (N) and peak resolution (R(s)) per column length and within a given total analysis time. The advantage of CSP prepared with core-shell silica is obvious from the viewp...Continue Reading

References

Mar 1, 2011·Journal of Chromatography. a·Georges Guiochon, Fabrice Gritti

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Citations

Jun 21, 2014·Analytical and Bioanalytical Chemistry·Michael Lämmerhofer
Jan 15, 2013·Journal of Pharmaceutical and Biomedical Analysis·Ahmed A YounesYvan Vander Heyden
Aug 20, 2015·Chemical Society Reviews·Manoj B GawandeRajender S Varma
May 5, 2016·International Journal of Analytical Chemistry·Raffaella Preti
Jun 16, 2015·Journal of Pharmaceutical and Biomedical Analysis·Yi MinYukui Zhang
May 10, 2016·Journal of Pharmaceutical and Biomedical Analysis·Sven DeclerckDebby Mangelings
May 7, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaoli ZhangYanping Shi
Jul 28, 2016·Journal of Chromatography. a·Darshan C PatelZachary S Breitbach
Aug 2, 2017·Electrophoresis
Mar 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chiara FanaliSalvatore Fanali
May 14, 2019·Electrophoresis·Salvatore Fanali, Bezhan Chankvetadze
May 20, 2014·Journal of Chromatography. a·Fabrice Gritti, Georges Guiochon
Nov 9, 2018·Analytical Chemistry·Valentina D'AtriDavy Guillarme

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