Comparative hydrocarbon utilization by hydrophobic and hydrophilic variants of Pseudomonas aeruginosa

Journal of Applied Microbiology
Christian ObuekweEsmaeil S Al-Saleh

Abstract

To investigate hydrocarbon degradation by hydrophobic, hydrophilic and parental strains of Pseudomonas aeruginosa. Partitioning of hydrocarbon-degrading P. aeruginosa strain in a solvent/aqueous system yielded hydrophobic and hydrophilic fractions. Exhaustive partitioning of aqueous-phase cells yielded the hydrophilic variants (L), while sequential fractionation of the hydrophobic phase cells yielded successive fractions exhibiting increasing cell-surface hydrophobicity (CSH). In hydrocarbon adherence assays (bacterial attachment to hydrocarbon), L had a value of 20%, which increased from 61.7% in first hydrophobic fraction (H(1)) to 72.2% in the third (H(3)). Crude oil degradation by L was 70%, but increased from 82% in H(1) to 93% in H(3). L variant produced most exopolysaccharides and reduced surface tension from about 73 to 49 mN m(-1). Rhamnolipid production was highest in L, but was not detected in all crude oil cultures. Hydrophobic subpopulations of hydrocarbon-degrading P. aeruginosa exhibited greater hydrocarbon-utilizing ability than hydrophilic ones, or the parental strain. Results demonstrate that a population of P. aeruginosa consists of cells with different CSH which affect hydrocarbon utilization. This potential...Continue Reading

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Citations

Oct 4, 2011·Applied Microbiology and Biotechnology·Hassan AbbasnezhadJulia M Foght
Feb 22, 2012·World Journal of Microbiology & Biotechnology·Łukasz ChrzanowskiKatarzyna Czaczyk
Jan 16, 2014·Indian Journal of Microbiology·Ewa KaczorekAndrzej Olszanowski
Jul 12, 2014·Chemosphere·Esmaeil Al-Saleh, Abrar Akbar
Jun 20, 2020·Colloids and Surfaces. B, Biointerfaces·Guruprakash Subbiahdoss, Erik Reimhult

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