Comparative immunohistochemical characterization of interstitial cells in the urinary bladder of human, guinea pig and pig

Histochemistry and Cell Biology
Clara SteinerJochen Neuhaus

Abstract

Interstitial cells (ICs) are thought to play a functional role in urinary bladder. Animal models are commonly used to elucidate bladder physiology and pathophysiology. However, inter-species comparative studies on ICs are rare. We therefore analyzed ICs and their distribution in the upper lamina propria (ULP), the deeper lamina propria (DLP) and the detrusor muscular layer (DET) of human, guinea pig (GP) and pig. Paraffin slices were examined by immunohistochemistry and 3D confocal immunofluorescence of the mesenchymal intermediate filament vimentin (VIM), alpha-smooth muscle actin (αSMA), platelet-derived growth factor receptor alpha (PDGFRα) and transient receptor potential cation channel A1 (TRPA1). Image stacks were processed for analysis using Huygens software; quantitative analysis was performed with Fiji macros. ICs were identified by immunoreactivity for VIM (excluding blood vessels). In all species ≥ 75% of ULP ICs were VIM+/PDGFRα+ and ≥ 90% were VIM+/TRPA1+. In human and pig ≥ 74% of ULP ICs were VIM+/αSMA+, while in GP the percentage differed significantly with only 37% VIM+/αSMA+ ICs. Additionally, over 90% of αSMA+ ICs were also TRPA1+ and PDGFRα+ in human, GP and pig. In all three species, TRPA1+ and PDGFRα+ ICs ...Continue Reading

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Citations

Nov 2, 2019·Physiological Reviews·Karel TalaveraBernd Nilius
May 5, 2018·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Aug 6, 2020·International Journal of Molecular Sciences·Jochen NeuhausFrank Peter Berger

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Methods Mentioned

BETA
fluorescence microscopy
electron microscopy

Software Mentioned

GraphPad Prism
cellSens
Photoshop
Huygens
ZEN Black
Fiji

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