Comparative in vitro efficacies of ethanol-, EDTA- and levofloxacin-based catheter lock solutions on eradication of Stenotrophomonas maltophilia biofilms

Journal of Medical Microbiology
B Passerini de RossiM Franco

Abstract

The aim of this study was to compare the in vitro activity of ethanol, EDTA and levofloxacin (Levo), alone or in combination, on biofilms of Stenotrophomonas maltophilia recovered from patients with catheter-related bloodstream infections (CRBSIs) at a university hospital in Argentina. First, 24 and 48 h biofilms were formed in microtitre plates and challenged with 25 or 40 % ethanol for 1 h. Biofilms, of the 14 local isolates and from the reference strain K279a, were eradicated after both treatments as shown by plate counts and the regrowth technique. Second, 24 h biofilms of all isolates were established in silicone catheter segments and challenged with 25 or 40 % ethanol, Levo (2.5 mg ml(-1)), EDTA (30 mg ml(-1)), 25 % ethanol-EDTA or Levo-EDTA for 1, 3 and 24 h. Viable counts of biofilms treated for 1 h with 25 or 40 % ethanol or 25 % ethanol-EDTA were under the limit of detection. Killing of biofilms by Levo or Levo-EDTA was gradual and it was only after 24 h of treatment that no differences could be seen between the effects of these catheter lock solutions (CLSs) and those of ethanol (P>0.05). Levo-EDTA, in combination, did not act synergistically against biofilms. After 24 h of exposure, EDTA did not eradicate biofilms b...Continue Reading

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Citations

Jan 1, 2014·Plastic and Reconstructive Surgery·James D KretlowShayan A Izaddoost
Feb 15, 2014·The Journal of Antimicrobial Chemotherapy·David W KubiakSophia Koo
Sep 22, 2015·Frontiers in Microbiology·Carlos A GarcíaBeatriz N Passerini de Rossi
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Jun 25, 2019·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Anne-Marie ChaftariIssam Raad
Apr 9, 2015·Medical Mycology·Yong LiaoRongya Yang
Oct 30, 2019·Expert Review of Anti-infective Therapy·Samantha Flores-TreviñoElvira Garza-González

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