Comparative in vitro phagocytosis and F-actin polymerization of bovine neonatal neutrophils

Zentralblatt für Veterinärmedizin. Reihe A
F MoiolaR D Zwahlen


Analysis of neonatal neutrophil (PMN) functions should help to reveal factors which could contribute to the impaired host defense system of neonates. We analysed functional parameters of PMN from newborn calves (N-PMN) and adult bovines (A-PMN): cellular volume and F-actin content upon stimulation with complement factors, by cytofluorometry and phagocytosis of E. coli 78:80B with a colorimetric assay. Polymerization of F-actin was rapid in both N- and A-PMN, but reached higher levels in N-PMN. N-PMN are significantly smaller than A-PMN throughout the whole activation time. Percentage of phagocytosing PMN, the rate of phagocytosis, and the rate of killing are similar between A- and N-PMN after opsonization of bacteria with adult serum (AS). Opsonization with newborn serum (NS) reduced all three examined parameters: in A-PMN more (P < 0.001) than in N-PMN (P < 0.05). However, when phagocytosis was compared following age-matched opsonization (N-PMN and NS; A-PMN and AS), N-PMN phagocytosed less (P < 0.001) bacteria per PMN than A-PMN. Additionally, steroidal (dexamethasone) and non-steroidal (phenylbutazone) anti-inflammatory drugs inhibited phagocytosis by N-PMN less than by A-PMN. Higher relative F-actin content of N-PMN can be ...Continue Reading


Nov 1, 1976·The British Veterinary Journal·S HudsonE Payne
May 1, 1992·The Journal of Surgical Research·J A FreischlagW S Moore
Nov 1, 1992·Pediatric Research·P N BochslerD O Slauson
Jul 1, 1991·Agents and Actions·D A LomasR A Stockley
Jan 1, 1991·Pharmaceutical Research·J F ScieszkaM J Cho
Feb 1, 1991·Pediatric Research·P RuefO Linderkamp
May 12, 1989·Journal of Immunological Methods·R Bellinati-PiresM M Carneiro-Sampaio
May 1, 1986·Veterinary Research Communications·P A HenricksF P Nijkamp
Aug 1, 1970·Archives of Disease in Childhood·J F Stevens
Jul 5, 1983·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P J England
Jan 1, 1984·Survey and Synthesis of Pathology Research·R BortolussiH P Seeliger
Feb 1, 1982·The Journal of Infectious Diseases·D M WilliamsC V Sumaya
Oct 1, 1980·Pediatric Research·R D Christensen, G Rothstein


Jul 1, 1998·Veterinary Immunology and Immunopathology·C MengeL H Wieler
Jan 17, 2015·Veterinary Immunology and Immunopathology·Hussain A AlabdullahRobert H Mealey
Nov 17, 2001·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·B R Hüsler, J W Blum
Mar 27, 2001·European Journal of Clinical Investigation·R KrauseC Wenisch
Dec 29, 1998·The Journal of Cell Biology·D A SchaferJ A Cooper

Related Concepts

Related Feeds

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.