PMID: 7024780Jul 7, 1981Paper

Comparative inactivation and inhibition of the anomerase and isomerase activities of phosphoglucose isomerase

Molecular and Cellular Biochemistry
E E Howell, K J Schray

Abstract

Several metabolic compounds have been found to be competitive inhibitors of the anomerase activity of phosphoglucose isomerase (EC 5.3.1.9).Ki values for erythrose 4-phosphate, 6-phosphogluconate, and fructose 1,6-bisphosphate for the anomerase reaction are 0.32 muM, 21 muM, and 84 muM respectively at 0 degree and pH 8.2. A significant difference between the fructose 1,6-bisphosphate inhibition constants for both activities was found (Ki(isomerase) = 800 muM and Ki(anomerase) = 140 muM). Also the Km values for both activities were found to be significantly different (Km(isomerase) = 140 muM and Km(anomerase) = 3.6 muM). Attempts to independently alter the anomerase to isomerase activity ratio through protein modification yielded mixed results. While several modifying reagents destroyed the catalytic activities at identical rates, inactivation by iodoacetamide or pyridoxal 5' phosphate sensitized photo-oxidation displayed differential initial effects on the two activities with the anomerase activity being the less affected. These data support the theory that an imidazole residue is catalytically important for isomerization, but less so for anomerization.

References

Jul 1, 1978·Archives of Biochemistry and Biophysics·K J Schray, E E Howell
Jan 1, 1979·Archives of Biochemistry and Biophysics·K J Schray, E E Howell
Oct 24, 1977·Biochemical and Biophysical Research Communications·D R GibsonF C Hartman
Sep 1, 1976·European Journal of Biochemistry·P BruchR W Gracy
Mar 1, 1974·Hoppe-Seyler's Zeitschrift für physiologische Chemie·B Wurster, B Hess
Apr 1, 1973·Hoppe-Seyler's Zeitschrift für physiologische Chemie·B Wurster, B Hess
Jul 16, 1973·European Journal of Biochemistry·P BruchE A Noltmann
Mar 16, 1971·Biochemistry·K J Schray, I A Rose
Mar 1, 1967·The Biochemical Journal·J Zalitis, I T Oliver

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Citations

May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Diana ArsenievaConstance J Jeffery
Jun 3, 2006·Journal of Biomedical Materials Research. Part a·L M SiperkoW J Landis

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