Comparative investigations of the glucosinolate-myrosinase system in Arabidopsis suspension cells and hypocotyls

Plant & Cell Physiology
Sophie AlvarezSixue Chen

Abstract

Glucosinolates are secondary metabolites derived from amino acids. Upon hydrolysis by myrosinases, they produce a variety of biologically active compounds. In this study, the glucosinolate-myrosinase system was characterized in Arabidopsis suspension cells. A total of seven glucosinolates were identified and the myrosinase activity was determined. Plant suspension cells have been used as model systems in many areas of study. To investigate whether the glucosinolate-myrosinase system in suspension cells works similarly to that in planta, 10-day-old seedling hypocotyls were used for comparative studies. A total of 16 glucosinolates were identified in hypocotyls. The two types of samples were also treated with methyljasmonate (MeJA)--a signaling compound induced by herbivore attack and wounding to initiate plant defense processes. The glucosinolate levels and their responses to MeJA varied greatly with the age of the cells. Two-day-old cells were most responsive, with the levels of all seven glucosinolates induced by MeJA, while in 4-day-old cells only the levels of indole glucosinolates were increased. In hypocotyls, the levels of indole glucosinolates and aliphatic glucosinolates (especially 4-methylsulfinylbutyl- and 8-methylsu...Continue Reading

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Citations

Oct 15, 2014·Journal of Proteome Research·Huizhuan YanSixue Chen
Jul 25, 2009·Journal of Proteomics·Sophie AlvarezSixue Chen
Aug 14, 2009·The Plant Journal : for Cell and Molecular Biology·Yan HeSixue Chen
Jul 28, 2016·Plant Signaling & Behavior·Daniel VikBarbara Ann Halkier
Aug 7, 2018·Journal of Integrative Plant Biology·Annette PetersenBarbara Ann Halkier
May 12, 2017·Planta·Pedro Joaquín Sánchez-PujanteLorena Almagro
Jun 6, 2019·Frontiers in Plant Science·Shweta ChhajedSixue Chen
Oct 2, 2008·Journal of Agricultural and Food Chemistry·Maria Rosecler Miranda RossettoBeatriz Rosana Cordenunsi

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