Comparative lipid analysis in the normal and cancerous organoids of MDCK cells

Journal of Biochemistry
Hisayoshi YoshizakiEtsuko Kiyokawa

Abstract

Epithelial organs are made of a well-polarized monolayer of epithelial cells, and their morphology is maintained strictly for their proper functioning. The roles of lipids are not only to generate the membrane, but also to provide the specific domains for signal transduction, or to transmit signals as second messengers. By using a liquid chromatography-electrospray ionization mass spectrometry (LC-MS)/MS method, we here analyzed sphingolipids in MDCK cysts under various conditions. Our result showed that, compared to the three-dimensional cyst, the two-dimensional MDCK sheet is relatively enriched in sphingolipids. During cystogenesis, the contents of sphingomyelin (SM) and lactocylceramide (LacCer)-but, none those of ceramide, hexocylceramide, or GM3-are altered depending on their acyl chains. While the total SM is decreased more efficiently by SMS-1 knockdown than by SMS-2 knockdown, depletion of SMS-2, but not SMS-1, inhibits cyst growth. Finally upon the switching on of activated K-Ras expression which induces luminal cell filling, ceramide and LacCer are increased. Our parallel examinations of the microarray data for mRNA of sphingolipid metabolic enzymes failed to fully explain the remodelling of the sphingolipids of MDCK...Continue Reading

References

Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H G HallM J Bissell
Jun 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·T AkagiH Hanafusa
Jul 3, 2002·Nature Reviews. Molecular Cell Biology·Lucy Erin O'BrienKeith E Mostov
Apr 5, 2003·The Journal of Biological Chemistry·Akiko Yamaji-HasegawaToshihide Kobayashi
Dec 26, 2003·Biophysical Journal·Reiko IshitsukaToshihide Kobayashi
Apr 21, 2005·The Journal of Biological Chemistry·Etsuko KiyokawaToshihide Kobayashi
Sep 20, 2005·Cancer Cell·Matthew J PaszekValerie M Weaver
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Yusuf A Hannun, Lina M Obeid
Jun 21, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Tiruneh K HailemariamXian-Cheng Jiang
Jul 16, 2008·The Journal of Biological Chemistry·Francisco Leocata NietoNorma B Sterin-Speziale
Aug 8, 2008·Molecular Biology of the Cell·Teruko NishiokaMichiyuki Matsuda
Mar 17, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Jing LiuXian-Cheng Jiang
Nov 17, 2009·Nature Methods·Kohei NishimuraMasato Kanemaki
Jan 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Julio L SampaioAndrej Shevchenko
Jun 2, 2011·Cold Spring Harbor Perspectives in Biology·Kai Simons, Julio L Sampaio
Jan 11, 2012·Journal of Lipid Research·Liana C SilvaAnthony H Futerman
Feb 2, 2012·The Journal of Biological Chemistry·Junichi IkenouchiMasato Umeda
Jul 26, 2012·The Journal of Biological Chemistry·Atsuro SakuraiEtsuko Kiyokawa
Jun 27, 2013·The Journal of Biological Chemistry·Hiroyuki NakamuraToshihiko Murayama
Jun 25, 2014·Journal of Biochemistry·Etsuko Kiyokawa, Hiroshi Minato

❮ Previous
Next ❯

Citations

Aug 20, 2019·Journal of Molecular Biology·Joan Bestard-EscalasGwendolyn Barceló-Coblijn
Aug 22, 2021·Cellular Signalling·Makoto Taniguchi, Toshiro Okazaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.