Comparative Metabolic Phenotyping of Tomato (Solanum lycopersicum ) for the Identification of Metabolic Signatures in Cultivars Differing in Resistance to Ralstonia solanacearum

International Journal of Molecular Sciences
Dylan R ZeissIan A Dubery

Abstract

Tomato (Solanum lycopersicum) is an important dietary source which contains numerous bioactive phytochemicals. Active breeding programs constantly produce new cultivars possessing superior and desirable traits. However, the underlying molecular signatures that functionally describe these traits are yet to be elucidated. Thus, in this study we used an untargeted metabolomic approach to describe differential metabolic profiles of four cultivars described as having high to intermediate resistance to Ralstonia solanacearum. Metabolites were methanol-extracted from leaves, stems and root tissues and analyzed by liquid chromatography coupled with high definition mass spectrometry. Multivariate data analysis revealed cultivar-related differential metabolic phenotypes. A total of 41 metabolites were statistically selected and annotated, consisting of amino acids, organic acids, lipids, derivatives of cinnamic acid and benzoic acids, flavonoids and steroidal glycoalkaloids which were especially prominent in the two highly resistant cultivars. Interestingly, the less resistant cultivars had various fatty acid derivatives in root extracts that contributed to the differentiated metabolic signatures. Moreover, the metabolic phenotype of the...Continue Reading

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Citations

Aug 17, 2019·International Journal of Molecular Sciences·Dylan R ZeissIan A Dubery
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Uyory ChoeLiangli Lucy Yu
Sep 17, 2020·Journal of Agricultural and Food Chemistry·Keabetswe MasikeMaria A Stander

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Methods Mentioned

BETA
PCA
SGA
glycosylation

Software Mentioned

MarkerLynx XS TM
ApexPeakTrack
MarkerLynx
SIMCA
Markerlynx XS

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