PMID: 3744195May 1, 1986Paper

Comparative metabolism of linamarin and amygdalin in hamsters

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
R A FrakesC C Willhite

Abstract

Rates of cyanide liberation resulting from hydrolysis of the cyanogenic glycosides linamarin, amygdalin and prunasin by a crude beta-glucosidase prepared from hamster caecum were studied in vitro. In addition, hamster blood cyanide and thiocyanate concentrations were determined at 0.5, 1, 2, 3 and 4 hr after an oral dose of 0.44 mmol linamarin or amygdalin/kg body weight. Plots of cyanide liberated v. time for linamarin and prunasin yielded straight lines. A similar plot for amygdalin was curvilinear, with the rate of cyanide release increasing with time. At 10(-3) M substrate concentrations, the average rates of hydrolysis of prunasin, amygdalin and linamarin were 1.39, 0.57 and 0.13 nmol/min/mg protein, respectively. Lineweaver-Burk plots yielded apparent Km and Vmax values of 3.63 X 10(-5) M and 0.35 nmol/min/mg protein, respectively, for amygdalin, and 7.33 X 10(-3) M and 1.04 nmol/min/mg protein, respectively, for linamarin. Blood cyanide concentrations following amygdalin treatment reached their highest level (130 nmol/ml) 1 hr after dosing and remained elevated until 3 hr after treatment. Blood cyanide concentrations following linamarin treatment reached their highest level (116 nmol/ml) after 3 hr and then declined imme...Continue Reading

References

Sep 1, 1978·Annals of Internal Medicine·R T Dorr, J Paxinos
Jan 1, 1979·Die Naturwissenschaften·E E Conn
Feb 1, 1977·Canadian Journal of Physiology and Pharmacology·M D BarrettA Zitnak
Mar 19, 1982·Science·C C Willhite
Mar 1, 1982·Archives of Toxicology·A G RauwsA Timmerman

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Citations

Feb 1, 1988·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M A WalligC C Willhite
Sep 25, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·N P Okolie, R O Omoigberale
Mar 24, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Roman A BlahetaEva Juengel
May 19, 2009·Disease-a-month : DM·Donald G Barceloux
Sep 3, 2008·Risk Analysis : an Official Publication of the Society for Risk Analysis·C R KirmanR Deskin
Sep 18, 2014·Acta Cirúrgica Brasileira·Rondon Tosta RamalhoPedro Carvalho Cassino
Nov 1, 2002·The Veterinary Record·B C McGorum, R A Anderson
Apr 11, 2019·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Tanja Schwerdtle

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