Comparative Metabolomic Analysis of the Cambium Tissue of Non-transgenic and Multi-Gene Transgenic Poplar (Populus × euramericana 'Guariento')

Frontiers in Plant Science
Kun NingXiaohua Su

Abstract

Poplar, a model for woody plant research, is the most widely distributed tree species in the world. Metabolites are the basis of phenotypes, allowing an intuitive and effective understanding of biological processes and their mechanisms. However, metabolites in non-transgenic and multi-gene transgenic poplar remains poorly characterized, especially in regards of the influences on quantity and in the analysis of the relative abundance of metabolites after the introduction of multi stress-related genes. In this study, we investigated the cambium metabolomes of one non-transgenic (D5-0) and two multi-gene (vgb, SacB, ERF36, BtCry3A, and OC-I) transgenic lines (D5-20 and D5-21) of hybrid poplar (Populus × euramericana 'Guariento') using both gas chromatography-mass spectrometry (GC-MS) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). We aimed to explore the effects of the exogenous genes on metabolite composition and to screen out metabolites with important biological functions. Finally, we identified 239 named metabolites and determined their relative abundance. Among these, 197 metabolites had a different abundance across the three lines. These methabolites spanned nine primary and 44 secondary me...Continue Reading

References

Apr 1, 1994·The Plant Cell·I. M. Van Der MeerSCM. Smeekens
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·John D Storey, Robert Tibshirani
Dec 17, 2005·Journal of Experimental Botany·Nigel M Crawford
Feb 14, 2006·The American Journal of Clinical Nutrition·Sidney M Morris
Feb 14, 2006·Transgenic Research·N FerryA M R Gatehouse
May 1, 2007·Current Opinion in Plant Biology·Motoaki SekiKazuo Shinozaki
May 27, 2008·Plant Physiology·Ayako NishizawaShigeru Shigeoka
Jan 21, 2009·Annual Review of Plant Biology·Kazuki Saito, Fumio Matsuda
Jan 24, 2009·Current Opinion in Plant Biology·Joost J B Keurentjes
Aug 22, 2009·Tree Physiology·Annie DeslauriersLaura Traversi
Mar 5, 2011·Annual Review of Plant Biology·Maor Bar-Peled, Malcolm A O'Neill
Oct 30, 2012·Current Opinion in Biotechnology·Jerzy Adamski, Karsten Suhre
May 17, 2013·Metabolomics : Official Journal of the Metabolomic Society·Hannes DoerflerWolfram Weckwerth
Jul 31, 2014·Journal of Integrative Plant Biology·Guorun QuJianxin Shi
Jan 1, 2015·Current Opinion in Lipidology·Helena GibbonsLorraine Brennan
Feb 26, 2015·Plant Science : an International Journal of Experimental Plant Biology·Julia Annika RichterChristian Zörb
Dec 26, 2015·Metabolic Engineering·Lisa Maria DerschChristoph Wittmann
Apr 6, 2016·Acta Biochimica Et Biophysica Sinica·Ziqing ChenShengce Tao
Sep 21, 2016·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Tiago F JorgeCarla António
Apr 11, 2018·Scientific Reports·Anna MichalettiSara Rinalducci
May 3, 2018·Frontiers in Plant Science·Mónica EscandónMaría Jesús Cañal

❮ Previous
Next ❯

Citations

Sep 10, 2019·Mass Spectrometry Reviews·Ana Margarida RodriguesCarla António
Jul 11, 2020·The New Phytologist·Ilka N AbreuThomas Moritz

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCA
MDA
electrophoresis

Software Mentioned

SIMCA
Metabolon

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.