Comparative Metatranscriptomics of Wheat Rhizosphere Microbiomes in Disease Suppressive and Non-suppressive Soils for Rhizoctonia solani AG8

Frontiers in Microbiology
Helen L HaydenPauline M Mele

Abstract

The soilborne fungus Rhizoctonia solani anastomosis group (AG) 8 is a major pathogen of grain crops resulting in substantial production losses. In the absence of resistant cultivars of wheat or barley, a sustainable and enduring method for disease control may lie in the enhancement of biological disease suppression. Evidence of effective biological control of R. solani AG8 through disease suppression has been well documented at our study site in Avon, South Australia. A comparative metatranscriptomic approach was applied to assess the taxonomic and functional characteristics of the rhizosphere microbiome of wheat plants grown in adjacent fields which are suppressive and non-suppressive to the plant pathogen R. solani AG8. Analysis of 12 rhizosphere metatranscriptomes (six per field) was undertaken using two bioinformatic approaches involving unassembled and assembled reads. Differential expression analysis showed the dominant taxa in the rhizosphere based on mRNA annotation were Arthrobacter spp. and Pseudomonas spp. for non-suppressive samples and Stenotrophomonas spp. and Buttiauxella spp. for the suppressive samples. The assembled metatranscriptome analysis identified more differentially expressed genes than the unassembled ...Continue Reading

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Citations

Jan 17, 2020·Plant Disease·Jay Ram LamichhaneJean-Noël Aubertot
Aug 29, 2020·Environmental Monitoring and Assessment·Swati MauryaSeema Makhija
Oct 6, 2020·Frontiers in Microbiology·Olubukola Oluranti BabalolaAyansina S Ayangbenro
Oct 15, 2019·Frontiers in Genetics·Migun ShakyaPatrick S G Chain
Mar 9, 2021·Computational and Structural Biotechnology Journal·Vanessa N KavamuraTim H Mauchline
Apr 12, 2021·Journal of Microbiological Methods·P PriyaK Harikrishnan

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Datasets Mentioned

BETA
SRP126206
SRR6349879

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

RNASeq
SQLite
Hmmer
Transrate
Trinotate
BLAST
Bowtie
Trinity scripts
PFam
DIAMOND

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