Comparative microarray analysis of Rhipicephalus (Boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on Bos indicus and Bos taurus cattle.

BMC Genomics
Manuel Rodriguez-ValleWayne Jorgensen

Abstract

Rhipicephalus (Boophilus) microplus is an obligate blood feeder which is host specific to cattle. Existing knowledge pertaining to the host or host breed effects on tick transcript expression profiles during the tick - host interaction is poor. Global analysis of gene expression changes in whole R. microplus ticks during larval, pre-attachment and early adult stages feeding on Bos indicus and Bos taurus cattle were compared using gene expression microarray analysis. Among the 13,601 R. microplus transcripts from BmiGI Version 2 we identified 297 high and 17 low expressed transcripts that were significantly differentially expressed between R. microplus feeding on tick resistant cattle [Bos indicus (Brahman)] compared to R. microplus feeding on tick susceptible cattle [Bos taurus (Holstein-Friesian)] (p <or= 0.001). These include genes encoding enzymes involved in primary metabolism, and genes related to stress, defence, cell wall modification, cellular signaling, receptor, and cuticle formation. Microarrays were validated by qRT-PCR analysis of selected transcripts using three housekeeping genes as normalization controls. The analysis of all tick stages under survey suggested a coordinated regulation of defence proteins, proteas...Continue Reading

References

Feb 1, 1978·Experimental Parasitology·C I Reich, J Zorzópulos
Sep 1, 1975·Journal of Insect Physiology·R H Hackman
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jul 21, 1998·Annals of the New York Academy of Sciences·E K MwangiM Murray
Dec 11, 1999·Nucleic Acids Research·J QuackenbushJ Upton
Dec 22, 1999·Genetic Analysis : Biomolecular Engineering·P Willadsen
May 29, 2000·Trends in Genetics : TIG·P RiceA Bleasby
Jan 9, 2001·Archives of Biochemistry and Biophysics·F HornC Termignoni
Feb 24, 2001·Cellular and Molecular Life Sciences : CMLS·H Wang, P A Nuttall
Apr 5, 2001·Trends in Immunology·P B Armstrong
Jun 5, 2002·Insect Biochemistry and Molecular Biology·M F Rosa de LimaA Masuda
Oct 9, 2002·Genome Research·Jason E StajichEwan Birney
Oct 24, 2002·International Journal for Parasitology·Vishvanath NeneRichard Bishop
Sep 13, 2003·BMC Bioinformatics·Roman L TatusovDarren A Natale
Oct 23, 2003·Experimental & Applied Acarology·Ivan G HorakJames E Keirans
Nov 24, 2004·Biochimie·Sergio Daishi SasakiAparecida Sadae Tanaka
Jan 18, 2005·Experimental & Applied Acarology·Manuel Rodriguez ValleMarisdania Joglar Piñeiro
Feb 1, 2005·Insect Biochemistry and Molecular Biology·P M UntalanT W Pearson
Mar 23, 2005·Trends in Parasitology·Catherine A Hill, Stephen K Wikel
Apr 29, 2005·Insect Biochemistry and Molecular Biology·F D GuerreroV Nene
Aug 17, 2005·Insect Biochemistry and Molecular Biology·Ivo M B FrancischettiJosé M C Ribeiro
Dec 21, 2005·Journal of Medical Entomology·Robert J MillerJohn E George
Dec 21, 2005·Toxicon : Official Journal of the International Society on Toxinology·N A SteenP F Alewood
Jan 25, 2006·Insect Biochemistry and Molecular Biology·José M C RibeiroStephen K Wikel
Mar 2, 2006·Journal of Medical Entomology·Felix D GuerreroPeter Willadsen
Mar 23, 2006·Insect Biochemistry and Molecular Biology·Ryan O M RegoLibor Grubhoffer
Apr 8, 2006·Experimental Parasitology·Alessandra CiprandiCarlos Termignoni
Jun 8, 2006·The Journal of Veterinary Medical Science·Chie NakajimaMisao Onuma
Jun 30, 2006·Biochemical and Biophysical Research Communications·Cassia A LimaAparecida S Tanaka
Sep 29, 2006·Trends in Parasitology·Daniel E SonenshineJosé de la Fuente
Nov 1, 2006·BMC Genomics·Luna De Ferrari, Stuart Aitken
Nov 14, 2006·Nucleic Acids Research·Christian von MeringPeer Bork
Nov 25, 2006·Molecular and Biochemical Parasitology·Marta CitelliPedro L Oliveira

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Citations

Oct 1, 2011·Vector Borne and Zoonotic Diseases·Lesley Bell-SakyiJohn K Fazakerley
Dec 24, 2013·BioMed Research International·Marina PoparaJosé de la Fuente
Mar 19, 2013·International Journal for Parasitology·Christian StutzerChristine Maritz-Olivier
Feb 23, 2013·International Journal for Parasitology·Albert MulengaAdriana Mércia Guaratini Ibelli
Dec 14, 2011·Topics in Companion Animal Medicine·Robin B Gasser, Cinzia Cantacessi
Jan 3, 2016·Ticks and Tick-borne Diseases·A E Lew-Tabor, M Rodriguez Valle
Dec 3, 2014·Ticks and Tick-borne Diseases·Willem A van ZylChristine Maritz-Olivier
Oct 15, 2014·Parasite Immunology·N N JonssonC C Constantinoiu
May 31, 2011·Veterinary Immunology and Immunopathology·Adriana SeixasItabajara da Silva Vaz
Jun 28, 2011·Veterinary Parasitology·Laercio R Porto NetoWilliam Barendse
Apr 26, 2011·International Journal for Parasitology·Ricardo F Mercado-CurielKelly A Brayton
Dec 20, 2011·International Journal for Parasitology·Matthew I BellgardAla E Lew-Tabor
Jul 9, 2016·Scientific Reports·Kirat K ChandPeter G Noakes
Jan 13, 2018·Frontiers in Cellular and Infection Microbiology·Ala E TaborNicholas N Jonsson
Jun 24, 2017·Frontiers in Cellular and Infection Microbiology·Sarah I BonnetOlivier Duron

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Methods Mentioned

BETA
electrophoresis
chip
PCR

Software Mentioned

BlastX
RPSBLAST
R
Emboss
BLAST
Bioperl
Blastn
GISP

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