Comparative molecular analysis of Na+/H+ exchangers: a unified model for Na+/H+ antiport?

FEBS Letters
P Dibrov, L Fliegel

Abstract

Despite 30 years of study on Na+/H+ exchange, the molecular mechanisms of antiport remain obscure. Most challenging, the identity of amino acids involved in binding transported cations is still unknown. We review data examining the identity of residues that are involved in cation binding and translocation of prokaryotic and eukaryotic Na+/H+ antiporters. Several polar residues specifically distributed within or immediately adjacent to membrane spanning regions are implicated as being important. These key amino acids are conserved in prokaryotes and in some lower eukaryotic forms of the Na+/ H+ antiporter, despite their being dispersed throughout the protein and despite an overall low similarity in the linear sequence of these Na+/H+ antiporters. We suggest that this conservation of isolated residues (together with distances between them) reflects a general physicochemical mechanism of cation binding by exchangers. The binding could be based on coordination of the substrate cation by a crown ether-like cluster of polar atomic groups amino acids, as has been hypothesized by Boyer. Traditional screening for the extended, highly conserved linear protein sequences might not be applicable when searching for functional domains of ion ...Continue Reading

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Citations

Jul 10, 2007·Journal of Physiology and Biochemistry·J L LequericaB Soria
Jul 9, 1999·Biochimica Et Biophysica Acta·A FukudaY Tanaka
Sep 10, 2010·The Journal of Biological Chemistry·Eiko Nakamaru-OgisoTomoko Ohnishi
Jan 30, 2009·The Journal of Biological Chemistry·Brian L LeeLarry Fliegel
Oct 29, 2004·Molecular and Cellular Biology·You-Tzung ChenAllan Bradley
Mar 4, 2010·Microbiology and Molecular Biology Reviews : MMBR·Joaquín AriñoHana Sychrová
Jul 7, 2007·Canadian Journal of Physiology and Pharmacology·Jie DingLarry Fliegel
Jun 21, 2001·The Biochemical Journal·C A WiebeL Fliegel
Jan 15, 2008·Journal of Molecular and Cellular Cardiology·Larry Fliegel
Oct 16, 2007·Biochimica Et Biophysica Acta·Emily SlepkovLarry Fliegel
Dec 13, 2012·Biochemical and Biophysical Research Communications·Fabiola BaltierraErwin Krauskopf
Aug 11, 2005·Canadian Journal of Physiology and Pharmacology·Larry FliegelPaul G Young
Jul 10, 2013·The Journal of Biological Chemistry·Asad UllahLarry Fliegel
Mar 5, 2020·Molecular and Cellular Biochemistry·Xiuju LiLarry Fliegel
Jan 29, 2005·The Journal of Biological Chemistry·Emily R SlepkovLarry Fliegel
Jul 17, 2008·Molecular and Cellular Biochemistry·Heng Chen, Larry Fliegel
Jan 11, 2007·The Biochemical Journal·Emily R SlepkovLarry Fliegel
Mar 20, 2016·Molecular and Cellular Biochemistry·Asad UllahLarry Fliegel
Dec 28, 2002·Annals of the New York Academy of Sciences·Larry FliegelDyal N Singh
Mar 15, 2001·Biochimica Et Biophysica Acta·E PadanN Dover

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