Comparative mutagenesis of Escherichia coli strains with different repair deficiencies irradiated with 222-nm and 254-nm ultraviolet light

Mutation Research
Marcus Clauss, Norbert Grotjohann

Abstract

Photoinactivation and reversion to tryptophan prototrophy were studied in four Escherichia coli strains with different repair deficiencies. Cells were irradiated with 222-nm wavelength UV emitted by an excimer lamp and with 254-nm wavelength UV emitted by a low-pressure mercury lamp. Strain DSM 9494 (trp(-)uvrA(+)) turned out to be most resistant while the strain DSM 9495 (trp(-)uvrA(-)), which is defective in nucleotide-excision repair (NER) was most sensitive to both wavelengths. UV-fluence rates for a respective inactivation were twice as high for 222-nm wavelength UV than for 254-nm UV. No clear difference in efficiency of inactivation could be observed between the two wavelengths in strains DSM 9496 (trp(-)uvrA(+) pKM101) and DSM 9497 (trp(-)uvrA(-) pKM101). In general, more revertants were induced by 254-nm wavelength UV, which corroborates the hypothesis that a higher amount of DNA damage was induced by this wavelength than by 222-nm UV, except for DSM 9497 where no clear difference could be observed regarding the number of revertants induced by both wavelengths. This strain DSM 9497 has a high sensitivity to certain oxidative mutagens compared with other strains, which is indicative of formation of reactive oxygen speci...Continue Reading

References

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Citations

Aug 30, 2005·Biochemical and Biophysical Research Communications·Xiaoyan LuRandy Legerski

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