PMID: 3321052Dec 1, 1987Paper

Comparative mutational analysis of wild-type and stretched tRNA3(Leu) gene promoters

Proceedings of the National Academy of Sciences of the United States of America
P FabrizioG P Tocchini-Valentini

Abstract

We demonstrate that, when the yeast tRNA(3Leu) gene is stretched so that the distance between the two portions of the intragenic promoter is increased to 365 base pairs, the A and B blocks remain functional. Mutations in the A block, which show a weak phenotype when inserted in the wild type, exert a dramatic effect when inserted into the stretched gene. Experiments with extensively purified transcription factor tau indicate that the tau B-B block interaction is not influenced by A-B distance; only the ability of tau A to interact with A block sequences is affected, possibly because of the additional free-energy cost of forming a large loop of the intervening DNA.

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R E BakerB D Hall
May 25, 1985·Journal of Molecular Biology·E T WilsonK U Sprague
Jan 1, 1984·Nucleic Acids Research·M Sprinzl, D H Gauss
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Citations

Dec 20, 1992·Journal of Molecular Biology·B R BraunE P Geiduschek
Sep 1, 1997·Molecular and Biochemical Parasitology·V NakaarC Tschudi
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R E BakerB D Hall
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D L Chalker, S B Sandmeyer
Aug 11, 1995·Nucleic Acids Research·P FruscoloniG P Tocchini-Valentini
Feb 24, 2000·Nucleic Acids Research·M R Paule, R J White
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·S ChédinA Sentenac
Dec 3, 2004·RNA·Gianfranco Di SegniGlauco P Tocchini-Valentini
Jan 20, 1990·Journal of Molecular Biology·K Abravaya, L B Rothman-Denes
Jul 30, 2014·Frontiers in Genetics·Tohru Yoshihisa
Jun 3, 2000·Journal of Molecular Biology·G DieciS Ottonello
Jan 13, 2006·The Journal of Biological Chemistry·George A Kassavetis, David F Steiner
Jun 22, 2006·The Journal of Biological Chemistry·Elisa GuffantiGiorgio Dieci
Jan 21, 2003·The Journal of Biological Chemistry·Sabine JourdainOlivier Lefebvre
May 1, 1993·Molecular and Cellular Biology·J B EschenlauerD A Brow

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