Comparative mutational evaluation of multiple lung cancers by multiplex oncogene mutation analysis

Cancer Science
Yuta TakahashiShinichi Toyooka

Abstract

In patients presenting with synchronous or metachronous multiple lung cancer (MLC), it is important to distinguish between multiple primary lung cancer (MP) and intrapulmonary metastasis (IM). The present study was aimed at investigating the mutational profiles of synchronous/metachronous MLC and to compare the classification of paired tumors by multiplex gene mutation analysis with the histopathological evaluation. We carried out targeted sequencing of 20 lung cancer-related oncogenes using next-generation sequencing (NGS) in 82 tumors from 37 MLC patients who underwent surgical resection at our department. The patients were diagnosed as MP or IM cases based on the Martini and Melamed criteria, histopathological and gene mutational evaluations. Matching mutations between paired tumors was observed in 20 (54%) patients, who were diagnosed as IM cases by mutational evaluation. Patients who could not be clearly diagnosed by histopathological evaluation were classified as equivocal cases. Among the histopathological IM cases (n = 7), six (86%) were confirmed as IM cases also by mutational evaluation, and most of the paired tumors of these cases (n = 5) harbored multiple matching mutations. Among the histopathological MP cases (n =...Continue Reading

References

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Citations

Sep 1, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jason C ChangNatasha Rekhtman
Oct 30, 2020·Cancer Management and Research·Lishu ZhaoChunhong Hu
Feb 9, 2021·Frontiers in Oncology·Xiaohui WangLele Song
Oct 22, 2021·International Journal of Cancer. Journal International Du Cancer·Bregtje C M HermansUNKNOWN PALGA-group

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Methods Mentioned

BETA
surgical resection

Software Mentioned

GeneRead Targeted Enrichment Panel
R commander
R
EZR

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