Comparative pharmacokinetics of baicalin and wogonoside by liquid chromatography-mass spectrometry after oral administration of Xiaochaihu Tang and Radix scutellariae extract to rats

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Zhenyu ZhuYifeng Chai

Abstract

The aim of this study was to compare the pharmacokinetics of baicalin and wogonoside in rats following oral administration of Xiaochaihu Tang (Minor Radix Bupleuri Decoction) and Radix scutellariae extract. Thus, a specific LC-MS method was developed and validated for the determination of these flavonoids in the plasma of rats after oral administration Xiaochaihu Tang and Radix scutellariae extract. Chromatographic separation was performed on a Zorbax SB C(18) column (150mmx4.6mm, i.d.: 5microm) with 0.1% formic acid in water and acetonitrile by linear gradient elution. Baicalin, wogonoside and carbamazepine (internal standard, I.S.) were detected in select-ion-monitoring (SIM) mode with a positive electrospray ionization (ESI) interface. The following ions: m/z 447 for baicalin, m/z 461 for wogonoside and m/z 237 for the I.S. were used for quantitative determination. The calibration curves were linear over the concentration ranges from 0.1231 to 6.156microg mL(-1) for baicalin and 0.08832 to 4.416microg mL(-1) for wogonoside. The lower limit of detection (LLOD) based on a signal-to-noise ratio of 2 was 0.06155microg mL(-1) for baicalin and 0.04416microg mL(-1) for wogonoside. Intra-day and inter-day precisions (RSD%) were with...Continue Reading

References

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Citations

Dec 3, 2013·Journal of Pharmaceutical and Biomedical Analysis·Hang ZhaoSiwang Wang
Jan 21, 2014·Journal of Ethnopharmacology·Guang Yue SuChun Fu Wu
May 7, 2013·Journal of Pharmaceutical and Biomedical Analysis·Fangyuan GaoGuorong Fan
Aug 19, 2014·Biomedical Chromatography : BMC·Yifan ZhangXinhong Wang
Dec 24, 2013·The Journal of Pharmacy and Pharmacology·Guang Yue SuChun Fu Wu
Apr 17, 2015·Journal of Pharmaceutical and Biomedical Analysis·Balázs MagdaPál T Szabó
Jul 31, 2016·Journal of Pharmaceutical and Biomedical Analysis·Youquan CongYun Fang
Mar 25, 2018·Rapid Communications in Mass Spectrometry : RCM·Ting DuGuangde Yang
Sep 14, 2018·European Journal of Drug Metabolism and Pharmacokinetics·Ting HuangChengliang Zhang
Sep 16, 2017·Journal of Food and Drug Analysis·Tun-Pin HsuehTung-Hu Tsai
Aug 4, 2019·Pharmaceutics·Robert AncuceanuVlad Negulescu
Oct 9, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Huiling YuPengwei Zhao
Oct 21, 2019·Journal of Ethnopharmacology·Kuo ZhangChunfu Wu
Jun 2, 2021·Journal of Pharmaceutical and Biomedical Analysis·Qianqian ZhangHongzhu Guo

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