Comparative phenotypic and functional analyses of the effects of autologous plasma and recombinant human macrophage-colony stimulating factor (M-CSF) on porcine monocyte to macrophage differentiation

Veterinary Immunology and Immunopathology
Giulia FranzoniAnnalisa Oggiano

Abstract

Porcine monocyte-derived macrophages (moMΦ) have been employed as a model cell in numerous studies of the porcine immune system. However, the lack of a standardized method for moMΦ differentiation hampers the comparison of results coming from the use of different laboratory protocols. In this study we compared the use of varying concentrations of autologous plasma (10, 20 and 30% v/v) or recombinant human macrophage-colony stimulating factor (hM-CSF; 50, 100, and 200ng/ml) to differentiate porcine monocytes into macrophages. Changes in cell morphology and surface marker expression were assessed by confocal microscopy and flow cytometry. Macrophage differentiation was evaluated by analysing TNF-α response to LPS stimulation and determining cytokine secretion patterns under both basal conditions and after classical and alternative activation. The effects of the differentiation methods on metabolic activity and susceptibility to infection with the myelotropic African swine fever virus (ASFV) were also evaluated. Monocytes cultured using the different culture conditions tested augmented in dimension and cellular complexity, but increasing porcine plasma concentrations resulted in a dose dependent enhancement in granularity and a ma...Continue Reading

Citations

Feb 7, 2018·Developmental and Comparative Immunology·Carmen A SautterArtur Summerfield
May 1, 2021·Animals : an Open Access Journal From MDPI·Tania CartaGiulia Franzoni
May 20, 2018·Veterinary Immunology and Immunopathology·Jiye GaoHenk P Haagsman
May 25, 2021·Frontiers in Molecular Biosciences·Ping LinShou-Dong Guo

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