Comparative physico-chemical studies on purified trypsin inhibitors from the endosperm of barley, rye, and wheat

Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung
S Boisen

Abstract

The dominant trypsin inhibitors of the endosperm of barley, rye and wheat were investigated in a series of comparative studies. The barley and rye inhibitors showed a reaction of partial immunochemical identity, whereas no immunological cross-reactions between the wheat inhibitor and the inhibitors from barley and rye were detected. The molecular weight of the barley inhibitor is about 14,500, and of the rye and wheat inhibitors about 12,000. The three inhibitors had very similar amino acid compositions, but the wheat inhibitor seemed to contain nine disulfide bridges whereas the barley and rye inhibitor contain five and four, respectively. This may explain the higher stability of the wheat inhibitor towards proteolytic inactivation by pepsin and chymotrypsin. All three inhibitors were very stable to temperatures at 100 degrees C. The barley inhibitor was much less active against porcine trypsin, and especially human trypsin, than against bovine trypsin, whereas the inhibition by the rye and wheat inhibitor was less dependent on the origin of the enzyme.

References

May 1, 1979·The Proceedings of the Nutrition Society·I E Liener
Jul 1, 1969·European Journal of Biochemistry·J Mikola, E M Suolinna
Nov 1, 1961·Archives of Biochemistry and Biophysics·B F ERLANGERW COHEN
Mar 20, 1947·The Journal of General Physiology·M Kunitz
Jan 1, 1980·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J JensenH Doll

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.