Comparative Platelet Releasate Proteomic Profiling of Acute Coronary Syndrome versus Stable Coronary Artery Disease

Frontiers in Cardiovascular Medicine
P B MaguireMeinrad Gawaz

Abstract

Upon activation, platelets release a host of soluble and vesicular signals, collectively termed the "platelet releasate" (PR). The contents of this PR play a significant role in haemostasis, inflammation, and pathologic sequelae. Despite this, proteomic studies investigating the PR in coronary artery disease have not been performed. Here, we undertook a comparative label-free quantitative (LFQ) proteomic profiling of the 1 U/ml thrombin-induced PR from 13 acute coronary syndrome vs. 14 stable angina pectoris patients using a tandem mass spectrometry approach. Data are available via ProteomeXchange with identifier PXD009356. 318 PR proteins were identified across both cohorts with 9 proteins found to be differentially released, including tetranectin (CLEC3B), protein disulfide-isomerase-A3 (PDIA3), coagulation factor V (F5), and fibronectin (FN1). Strikingly, these 9 differential proteins were all associated with the gene ontology cellular component term "extracellular vesicle" and reduced levels of EVs were detected in the corresponding plasma of ST-segment elevation myocardial infarction (STEMI) patients. Network analysis revealed 3 proteins either reduced (F5; FN1) or absent (CLEC3B) in the PR of STEMI patients that are stron...Continue Reading

References

Jul 15, 1989·Thrombosis Research·C KluftI Clemmensen
Sep 11, 2002·Biochemical and Biophysical Research Communications·Sudawadee SuttitanamongkolAdrian R L Gear
Nov 2, 2002·Nature Medicine·Zaverio M Ruggeri
May 6, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Jianhui ZhuStephen E Epstein
Jun 21, 2003·BMJ : British Medical Journal·Ever D Grech, David R Ramsdale
Nov 1, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Masataka HoriuchiYasuhide Nakashima
Oct 19, 2004·Journal of the American College of Cardiology·Petra J Mateos-CáceresLuis A Rico
Dec 3, 2005·The Journal of Clinical Investigation·Meinrad GawazAndreas E May
Feb 17, 2007·Blood·Judith A CoppingerGerard Cagney
Nov 28, 2008·Blood·Giannoula Lakka KlementJudah Folkman
Jan 30, 2009·Bioinformatics·Davis J McCarthy, Gordon K Smyth
Feb 16, 2010·Cardiovascular Research·Julio Madrigal-MatuteJose Luis Martín-Ventura
Apr 24, 2010·Seminars in Thrombosis and Hemostasis·Rory R Koenen, Christian Weber
Oct 22, 2010·Blood·Karen P FongLawrence F Brass
Dec 16, 2011·Journal of Thrombosis and Haemostasis : JTH·L-M HolbrookJ M Gibbins
Aug 28, 2012·European Heart Journal·Kristian ThygesenUNKNOWN ESC Committee for Practice Guidelines (CPG)
May 4, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Patrick WijtenArjen Scholten
Aug 27, 2015·Advances in Experimental Medicine and Biology·Kristina N EkdahlBo Nilsson
Sep 24, 2015·Journal of Thrombosis and Haemostasis : JTH·H Heijnen, P van der Sluijs
Dec 2, 2015·Scientific Reports·Yanjia ChenWei Jin
Feb 18, 2016·Seminars in Thrombosis and Hemostasis·Madhumita Chatterjee, Tobias Geisler
Jun 10, 2016·Archives of Medical Science : AMS·Anggoro Budi HartopoBudi Yuli Setianto
Jun 23, 2016·Proteomics·Paulina B SzklannaPatricia B Maguire
Dec 9, 2016·Platelets·Oliver PagelRené P Zahedi
Apr 22, 2017·European Heart Journal·Madhumita ChatterjeeMeinrad Gawaz
Jul 5, 2017·Journal of the American Heart Association·Meiping RenJianbo Wu
May 5, 2018·Metabolism: Clinical and Experimental·Anouar Hafiane, Stella S Daskalopoulou
Oct 16, 2018·Proteomics. Clinical Applications·Paulina B SzklannaPatricia B Maguire

❮ Previous
Next ❯

Citations

May 6, 2021·International Journal of Molecular Sciences·Olga ShevchukAlbert Sickmann

❮ Previous
Next ❯

Datasets Mentioned

BETA
PXD009356

Methods Mentioned

BETA
proteomic profiling
protein folding

Software Mentioned

STRING
Panther
Andromeda
Perseus

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.