Comparative profiling of the mammalian mitochondrial proteome: multiple aconitase-2 isoforms including N-formylkynurenine modifications as part of a protein biomarker signature for reactive oxidative species

Journal of Proteome Research
Christian HunzingerAndré Schrattenholz

Abstract

The activity of mitochondria induces, as a byproduct, a variety of post-translational modifications in associated proteins, which have functional downstream consequences for processes such as apoptosis, autophagy, and plasticity; e.g., reactive oxygen species (ROS), which induce N-formyl-kynurenine from oxidized tryptophans in certain mitochondrial proteins which are localized in close spatial proximity to their source. This type of fast molecular changes has profound influence on cell death and survival with implications in a number of pathologies. The quantitative and differential analysis of bovine heart mitochondria by four 2D-PAGE methods, including 2D-PAGE with high-resolution IEF as first dimension, revealed that due to limited resolution, those methods employing blue native-, tricine-urea-, and 16-BAC-PAGE as the first dimension are less applicable for the differential quantitative analysis of redundant protein spots which might give insight into post-translational modifications that are relevant in age- and stress-related changes. Moreover, 2D-PAGE with high resolution IEF was able to resolve a surprisingly large number of membrane proteins from mitochondrial preparations. For aconitase-2, an enzyme playing an importan...Continue Reading

Citations

Apr 30, 2011·The Journal of Biological Chemistry·Tina M DreadenBridgette A Barry
Jun 2, 2010·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Renato M P AlvesFrancisco Amado
Mar 20, 2012·Journal of Hematology & Oncology·Yujie Jiang, Xin Wang
Aug 8, 2007·Analytical and Bioanalytical Chemistry·Ralf J BraunMarius Ueffing
Mar 12, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Lerna UzasciRobert Cotter
Sep 13, 2008·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·M KlemmA Schrattenholz
Sep 5, 2008·Biogerontology·Custer C DeocarisRenu Wadhwa
Nov 20, 2012·Photosynthesis Research·Tina M Dreaden Kasson, Bridgette A Barry
Oct 22, 2008·Expert Review of Proteomics·D Brian FosterJennifer E Van Eyk
Jul 23, 2009·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·D J AntoineB K Park
Jun 25, 2008·Journal of Proteomics·Veronika Reisinger, Lutz Andreas Eichacker
Jan 25, 2008·Experimental Gerontology·Vukić SoskićAndré Schrattenholz
Mar 13, 2016·Neuropharmacology·D González EsquivelV Pérez de la Cruz
Dec 17, 2008·Mass Spectrometry Reviews·Thierry RabilloudMireille Chevallet
May 15, 2007·Journal of Cellular Biochemistry·Sara PadidarJanice E Drew
May 1, 2008·Biotechnology Journal·Diksha Dani, Norbert A Dencher
Feb 1, 2009·Proteomics. Clinical Applications·Deborah Penque
Sep 4, 2008·Proteomics·Ilka Wittig, Hermann Schägger
Apr 27, 2007·Annals of the New York Academy of Sciences·Norbert A DencherFrank Krause
Oct 17, 2009·Proteomics·Ingrid MillerElisabetta Gianazza
Mar 19, 2009·Mass Spectrometry Reviews·Yie Hou LeeMaxey C M Chung
Dec 3, 2011·Biochimica Et Biophysica Acta·Sascha RexrothNorbert A Dencher
Mar 2, 2010·Experimental Gerontology·Karlfried GroebeAndré Schrattenholz
Feb 18, 2010·Experimental Gerontology·Monika FrenzelNorbert A Dencher
Aug 4, 2012·PloS One·Tina M Dreaden KassonBridgette A Barry
Jun 21, 2011·International Journal of Molecular Sciences·Haider RazaNarayan G Avadhani
Sep 11, 2020·Free Radical Biology & Medicine·Stephanie BellmaineAline Zimmer
Aug 11, 2021·Chemistry : a European Journal·Zahra NasriKristian Wende
Aug 9, 2007·Chemical Reviews·Anna E Speers, Christine C Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms