Comparative proteome analysis of cellular proteins extracted from highly virulent Francisella tularensis ssp. tularensis and less virulent F. tularensis ssp. holarctica and F. tularensis ssp. mediaasiatica

Proteomics
Martin HubalekJ Stulík

Abstract

Francisella tularensis is the causative agent of the zoonotic disease tularemia. Four subspecies of this pathogen, namely ssp. tularensis, mediaasiatica, holarctica, and novicida are spread throughout the northern hemisphere. Although there are marked variations in their virulence to mammals, the subspecies are difficult to identify as they are closely genetically related. We carried out the comparative proteome analysis of cellular extracts from isolates representing the highly virulent subspecies tularensis, and the less virulent subspecies mediaasiatica and holarctica in order to identify new diagnostic markers and putative factors of virulence. We identified 27 protein spots that were either specifically present or at significantly higher abundance in ssp. tularensis strains, 22 proteins in ssp. mediaasiatica strains, and 26 proteins in ssp. holarctica strains. Subspecies tularensis-specific proteins might represent putative virulence factors. Of 27 identified tularensis-specific spots 17 represented charge and mass variants of proteins occurring in other subspecies, 7 spots were found to be present at higher abundance, and 3 spots were specifically present in tularensis strains. Amongst them, PilP protein, as a component n...Continue Reading

Citations

Jun 7, 2005·Biochimica Et Biophysica Acta·Richard L FeltsJohn J Tanner
Feb 24, 2006·Infection and Immunity·Erin E McClellandArturo Casadevall
May 19, 2006·Journal of Bacteriology·Jonathan Tabb SullivanGirija Ramakrishnan
Jun 30, 2009·BMC Infectious Diseases·Hana BandouchovaJiri Pikula
Jan 15, 2011·Acta Veterinaria Scandinavica·Hana BandouchovaJiri Pikula
May 17, 2015·Journal of Proteomics·Noemí BujánBeatriz Magariños
Dec 8, 2010·Proteomics. Clinical Applications·Henry J WindleDermot P Kelleher
Dec 1, 2017·Experimental & Applied Acarology·Dario PistonePaolo Lanfranchi
May 17, 2005·Expert Review of Proteomics·Celia G ZhangSandra L McCutchen-Maloney
May 17, 2005·Expert Review of Proteomics·Richard R DrakeStefan Gravenstein
Apr 20, 2017·Frontiers in Cellular and Infection Microbiology·Girija Ramakrishnan

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