Comparative Proteome-Wide Analysis of Bone Marrow Microenvironment of β-Thalassemia/Hemoglobin E.

Proteomes
Saranyoo PonnikornSuradej Hongeng

Abstract

β-thalassemia/Hb E is a global health issue, which is characterized by a range of clinical symptoms from a mild and asymptomatic anemia to severe disorders that require transfusions from infancy. Pathological mechanisms of the disease involve the excess of unmatched alpha globin and iron overload, leading to ineffective erythropoiesis and ultimately to the premature death of erythroid precursors in bone marrow (BM) and peripheral organs. However, it is unclear as to how BM microenvironment factors contribute to the defective erythropoiesis in β-thalassemia/Hb E patients. Here, we employed mass spectrometry-based comparative proteomics to analyze BM plasma that was collected from six β-thalassemia/Hb E patients and four healthy donors. We identified that the differentially expressed proteins are enriched in secretory or exosome-associated proteins, many of which have putative functions in the oxidative stress response. Using Western blot assay, we confirmed that atypical lipoprotein, Apolipoprotein D (APOD), belonging to the Lipocalin transporter superfamily, was significantly decreased in BM plasma of the tested pediatric β-thalassemia/Hb E patients. Our results highlight that the disease condition of ineffective erythropoiesis...Continue Reading

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Citations

Oct 26, 2021·Frontiers in Physiology·Diego Sanchez, Maria D Ganfornina

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Methods Mentioned

BETA
interaction prediction
nuclear translocation

Software Mentioned

Prism
STRING Search Tool for the Retrieval
BioTools
MeV experiment viewer
PepDetect
MeV
Secretome P2
MASCOT MS / MS Ion engine
DeCyder MS Differential Analysis
Panther

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