Comparative proteomic analysis between normal skin and keloid scar

The British Journal of Dermatology
C T OngT T Phan

Abstract

Keloids are pathological scars and, despite numerous available treatment modalities, continue to plague physicians and patients. Identification of molecular mediators that contribute to this fibrotic phenotype. Two-dimensional gel electrophoresis, MALDI-TOF, Mascot online database searching algorithm and Melanie 5 gel analysis software were employed for comparative proteomic analysis between normal skin (NS) and keloid scar (KS) tissue extracts. Seventy-nine protein spots corresponding to 23 and 32 differentially expressed proteins were identified in NS and KS, respectively. Isoforms of heat shock proteins, gelsolin, carbonic anhydrase and notably keratin 10 were strongly expressed in NS along with manganese superoxide dismutase, immune components, antitrypsin, prostatic binding protein and crystalline. Various classes of proteins were found either to be present or to be upregulated in keloid tissue: (i) inflammatory/differentiated keratinocyte markers: S100 proteins, peroxiredoxin I; (ii) wound healing proteins: gelsolin-like capping protein; (iii) fibrogenetic proteins: mast cell β-tryptase, macrophage migration inhibitory factor (MIF); (iv) antifibrotic proteins: asporin; (v) tumour suppressor proteins: stratifin, galectin-1...Continue Reading

References

Mar 1, 1977·Plastic and Reconstructive Surgery·R F DiegelmannI K Cohen
Feb 1, 1975·European Journal of Clinical Investigation·R D Craig
Jan 1, 1990·Annual Review of Cell Biology·J Massagué
Sep 1, 1989·The Journal of Pathology·S E KellyS Fleming
Jan 1, 1989·Connective Tissue Research·C W KischerP Scuderi
Jul 1, 1995·Journal of Pediatric Surgery·S M AlaishI K Cohen
Dec 14, 1995·Nature·N L PerilloL G Baum
Mar 1, 1996·The Journal of Surgical Research·M CalderonA J Banes
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A ShevchenkoM Mann
Jul 11, 1998·Molecular Cell·H HermekingB Vogelstein
Mar 19, 1999·Molecular and Cellular Biology·J M ParamioJ L Jorcano
Apr 2, 1999·Seminars in Cancer Biology·W S el-Deiry
May 8, 1999·The Journal of Investigative Dermatology·A B Katz, L B Taichman
Aug 3, 1999·The Journal of Experimental Medicine·G A RabinovichY Chernajovsky
Aug 24, 1999·Annals of Plastic Surgery·T Y LeeM T Longaker
Oct 8, 1999·Plastic and Reconstructive Surgery·F B NiessenM Kon
Aug 19, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·L WangM W Büchler
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C D ChungM C Miceli
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·G PellegriniM De Luca
Nov 17, 2001·The Journal of Investigative Dermatology·J GailitB L Gruber
Apr 23, 2003·Electrophoresis·Federica BoraldiDaniela Quaglino
May 3, 2003·The Journal of Clinical Investigation·Gillian S AshcroftToshinori Nakayama
May 6, 2003·Gastroenterology·Luca SantucciAntonio Morelli
Jul 4, 2003·The Journal of Investigative Dermatology·Chun-Ming HuangDe-chu C Tang
Aug 2, 2003·Nature Reviews. Neuroscience·Joyce Tombran-Tink, Colin J Barnstable
Sep 6, 2003·The Journal of Surgical Research·Wei ChenZhiyong Sheng
Nov 18, 2003·The Journal of Biological Chemistry·Jiale He, Linda G Baum
May 14, 2004·The Journal of Investigative Dermatology·Aziz GhaharyMichael Weinfeld
Jul 28, 2004·Archives of Dermatological Research·Diana V MessadiAnh D Le
Sep 18, 2004·The Journal of Investigative Dermatology·Josip BlonderJonathan C Vogel
Mar 4, 2005·The Journal of Biological Chemistry·Ehab H SarsourPrabhat C Goswami

❮ Previous
Next ❯

Citations

Mar 13, 2012·Archives of Dermatological Research·F Javad, P J R Day
Nov 22, 2013·BioMed Research International·Ju Hee LeeWon Jai Lee
Apr 24, 2014·Advances in Wound Care·Traci A Wilgus, Brian C Wulff
Jan 10, 2014·Archives of Dermatological Research·Soon Heum KimBokyung Kim
Dec 17, 2010·The Journal of Investigative Dermatology·Alvin W C ChuaToan T Phan
Oct 26, 2012·The Journal of Investigative Dermatology·Johanna CanadyAnja K Bosserhoff
Sep 11, 2014·The Journal of Investigative Dermatology·Claire da Silva SantosSerhiy Souchelnytskyi
Aug 16, 2011·Journal of the European Academy of Dermatology and Venereology : JEADV·G P Sidgwick, A Bayat
Jul 3, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jennifer M HahnDorothy M Supp
Apr 16, 2015·Journal of Medical Toxicology : Official Journal of the American College of Medical Toxicology·Keith K BurkhartDavid Jackson
Dec 20, 2015·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Yun-Shain LeeTai-Lan Tuan
Dec 15, 2017·Advances in Skin & Wound Care·Feras M GhazawiFatemeh Jafarian
Nov 30, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xinqiang HuangGabor Jarai
Feb 26, 2019·The American Journal of Dermatopathology·Enrique ArciniegasMarina Chopite
Jul 6, 2016·The British Journal of Dermatology·G C LimandjajaS Gibbs
May 21, 2020·Journal of Burn Care & Research : Official Publication of the American Burn Association·Xingtang NiuQing Tang
Dec 16, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Venkata ThulabanduRadhika P Atit
Dec 14, 2018·Physiological Measurement·Xi GaoRobert F Diegelmann
Jul 28, 2020·International Journal of Molecular Sciences·Toini PemmariTero A H Järvinen
Feb 18, 2020·Frontiers in Pharmacology·Xiaoxiao PangZhong Zheng
Jan 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Vera VorstandlechnerMichael Mildner
Jun 13, 2020·Frontiers in Cell and Developmental Biology·Grace C LimandjajaSusan Gibbs
Jul 1, 2013·Plastic and Reconstructive Surgery. Global Open·Chenyu HuangRei Ogawa
Feb 11, 2018·Experimental Dermatology·Nan-Lin Wu, Fu-Tong Liu
Feb 14, 2020·JPRAS Open·Ferdinand W Nangole, George W Agak
Jun 10, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Longwei LiuChenyu Huang
Jun 24, 2021·Scars, Burns & Healing·Chenyu Huang, Rei Ogawa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Cancer -Omics

A variety of different high-throughput technologies can be used to identify the complete catalog of changes that characterize the molecular profile of cohorts of tumor samples. Discover the latest insights gained from cancer 'omics' in this feed.