Comparative Proteomic Analysis of Carbonylated Proteins from the Striatum and Cortex of Pesticide-Treated Mice

Parkinson's Disease
Christina CoughlanJames R Roede

Abstract

Epidemiological studies indicate exposures to the herbicide paraquat (PQ) and fungicide maneb (MB) are associated with increased risk of Parkinson's disease (PD). Oxidative stress appears to be a premier mechanism that underlies damage to the nigrostriatal dopamine system in PD and pesticide exposure. Enhanced oxidative stress leads to lipid peroxidation and production of reactive aldehydes; therefore, we conducted proteomic analyses to identify carbonylated proteins in the striatum and cortex of pesticide-treated mice in order to elucidate possible mechanisms of toxicity. Male C57BL/6J mice were treated biweekly for 6 weeks with saline, PQ (10 mg/kg), MB (30 mg/kg), or the combination of PQ and MB (PQMB). Treatments resulted in significant behavioral alterations in all treated mice and depleted striatal dopamine in PQMB mice. Distinct differences in 4-hydroxynonenal-modified proteins were observed in the striatum and cortex. Proteomic analyses identified carbonylated proteins and peptides from the cortex and striatum, and pathway analyses revealed significant enrichment in a variety of KEGG pathways. Further analysis showed enrichment in proteins of the actin cytoskeleton in treated samples, but not in saline controls. These d...Continue Reading

References

Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
May 1, 1991·Archives of Otolaryngology--head & Neck Surgery·D A DeemsJ B Snow
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·A YoritakaY Mizuno
Sep 28, 1998·Annals of Neurology·P Jenner, C W Olanow
Aug 31, 2000·Neurobiology of Disease·Y ZhangT M Dawson
Oct 18, 2000·European Journal of Pharmacology·N Chen, M E Reith
Jul 5, 2001·Environmental Research·A PriyadarshiS S Priyadarshi
Nov 14, 2002·Neurotoxicology·Mona ThiruchelvamDeborah A Cory-Slechta
Nov 20, 2002·Chemical Research in Toxicology·Jonathan A Doorn, Dennis R Petersen
Jan 18, 2003·Vascular Pharmacology·Hartwig Wolburg, Andrea Lippoldt
Feb 27, 2003·Chemico-biological Interactions·Jonathan A Doorn, Dennis R Petersen
Nov 1, 2003·Science·Ted M Dawson, Valina L Dawson
Jun 13, 2006·Behavioural Brain Research·Jennifer L TillersonGary W Miller
Jul 25, 2006·European Journal of Pharmacology·D Allan ButterfieldRukhsana Sultana
Sep 22, 2006·Antioxidants & Redox Signaling·Dean P Jones
Jul 27, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·W Michael CaudleGary W Miller
Sep 15, 2007·Biochimica Et Biophysica Acta·Andrea Hartsock, W James Nelson
Oct 16, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Gary Wright Miller
Dec 21, 2007·Lancet Neurology·Anthony H V Schapira
Mar 1, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Richard M LoPachin, Terrence Gavin
Mar 18, 2008·Free Radical Biology & Medicine·Derek A Drechsel, Manisha Patel
Aug 8, 2008·American Journal of Physiology. Cell Physiology·Dean P Jones
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Apr 28, 2009·Biochimica Et Biophysica Acta·Benjamin J StewartDennis R Petersen
Jun 24, 2009·Chemical Research in Toxicology·James R RoedeDennis R Petersen
Jun 26, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tonya N TaylorGary W Miller
Jul 11, 2009·Environmental Health Perspectives·Beate R RitzJeff Bronstein
Oct 23, 2010·Journal of Pineal Research·Naveen Kumar SinghalMahendra Pratap Singh
Mar 16, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·James R RoedeDean P Jones
Apr 1, 2011·Chemical Research in Toxicology·Kristofer S FritzDennis R Petersen
Apr 21, 2011·European Journal of Epidemiology·Anthony WangBeate Ritz
Jun 21, 2011·Parkinson's Disease·Antje HaehnerHeinz Reichmann
Apr 17, 2012·Chemical Research in Toxicology·James J GalliganDennis R Petersen
Sep 8, 2012·Behavioural Brain Research·Michal ShelegJason R Richardson

❮ Previous
Next ❯

Citations

Nov 3, 2016·Journal of Lipid Research·Amy K Hauck, David A Bernlohr
May 17, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Colin C AndersonJames R Roede
Aug 24, 2019·Journal of the Science of Food and Agriculture·Zhanrui HuangJian Zhao

❮ Previous
Next ❯

Methods Mentioned

BETA
carbonylation
pull-down
Protein Carbonylation

Software Mentioned

DAVID
R
Database for Annotation , Visualization , and Integrated Disco...
Mascot
Image Lab

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

© 2021 Meta ULC. All rights reserved