Comparative proteomic analysis of Cronobacter sakazakii isolates with different virulences

Journal of Proteomics
Xin-Jun DuShuo Wang

Abstract

Cronobacter is a genus of widespread, opportunistic, foodborne pathogens that can result in serious illnesses in at-risk infants because of their immature immunity and high dependence on powdered formula, which is one of the foods most often contaminated by this pathogen. However, limited information is available regarding the pathogenesis and the specific virulence factors of this species. In this study, the virulences of 42 Cronobacter sakazakii isolates were analyzed by infecting neonatal SD rats. A comparison of the typing patterns of the isolates enabled groups with close relationships but that exhibited distinct pathogenesis to be identified. Among these groups, 2 strains belonging to the same group but showing distinct virulences were selected, and 2-DE was applied to identify differentially expressed proteins, focusing on virulence-related proteins. A total of 111 protein spots were identified using matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF-MS), and 89 were successfully identified. Further analysis suggested that at least 11 of these proteins may be involved in the pathogenesis of this pathogen. Real-time PCR was carried out to further confirm the differential exp...Continue Reading

References

Apr 21, 1999·Trends in Microbiology·D E Taylor
Jan 16, 2004·Lancet·M Chantal KandhaiMike van Schothorst
Mar 3, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D DrudyS Fanning
Mar 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·David S WeissDenise M Monack
Aug 2, 2007·Canadian Journal of Microbiology·Mamta Raghav, P K Aggarwal
Nov 6, 2007·Current Protein & Peptide Science·K Ananda KrishnaK R S Sambasiva Rao
Nov 21, 2008·Molecular Plant Pathology·Annie LauzierCarole Beaulieu
Aug 8, 2009·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M Friedemann
Dec 5, 2009·Foodborne Pathogens and Disease·Brendan HealySéamus Fanning
Dec 17, 2009·International Journal of Medical Microbiology : IJMM·Michael HeckerSusanne Engelmann
Jun 15, 2010·Applied and Environmental Microbiology·Kyumson KimSangryeol Ryu
Feb 8, 2011·Veterinary Microbiology·Duraisamy PonnusamyKenneth D Clinkenbeard
Mar 23, 2011·Applied and Environmental Microbiology·A A FrancoB D Tall
Jun 15, 2011·Veterinary Microbiology·Wei DangLi Sun
Nov 29, 2011·Molecular BioSystems·Cristian PirasPaola Roncada
Nov 21, 2012·PloS One·Susan JosephStephen J Forsythe
Dec 12, 2012·Molecular Immunology·Mohammad Reza Asadi KaramSaeid Bouzari
Feb 2, 2013·Applied Microbiology and Biotechnology·Jian WangShuo Wang
Mar 30, 2013·Journal of Perinatology : Official Journal of the California Perinatal Association·C J Hunter, J F Bean
May 28, 2013·Microbial Informatics and Experimentation·Susan JosephStephen Forsythe
Jun 12, 2013·Veterinary Microbiology·Qiangde DuanGuoqiang Zhu
Dec 18, 2013·The Journal of Hospital Infection·O Holý, S Forsythe
Dec 25, 2013·Infection and Immunity·Sarah E FranksShauna M McGillivray
Dec 25, 2013·Infection and Immunity·Amy M KingGerald L Murray
Jul 17, 2014·PloS One·Jinghua CuiZhigang Cui

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