Comparative proteomic analysis of differentially expressed proteins between peripheral sensory and motor nerves

Journal of Proteome Research
Qianru HeXiaosong Gu

Abstract

Peripheral sensory and motor nerves have different functions and different approaches to regeneration, especially their distinct ability to accurately reinervate terminal nerve pathways. To understand the molecular aspects underlying these differences, the proteomics technique by coupling isobaric tags for relative and absolute quantitation (iTRAQ) with online two-dimensional liquid chromatography tandem mass spectrometry (2D LC-MS/MS) was used to investigate the protein profile of sensory and motor nerve samples from rats. A total of 1472 proteins were identified in either sensory or motor nerve. Of them, 100 proteins showed differential expressions between both nerves, and some of them were validated by quantitative real time RT-PCR, Western blot analysis, and immunohistochemistry. In the light of functional categorization, the differentially expressed proteins in sensory and motor nerves, belonging to a broad range of classes, were related to a diverse array of biological functions, which included cell adhesion, cytoskeleton, neuronal plasticity, neurotrophic activity, calcium-binding, signal transduction, transport, enzyme catalysis, lipid metabolism, DNA-binding, synaptosome function, actin-binding, ATP-binding, extracellu...Continue Reading

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Citations

Oct 16, 2012·Clinical Biochemistry·David Cilia Vincenti, Graeme I Murray
Jan 22, 2013·Proteomics·Catarina FrancoAna Varela Coelho
Apr 30, 2013·Methods : a Companion to Methods in Enzymology·George E CraftAngus C Nairn
Jan 29, 2019·Frontiers in Neuroscience·Qianru HeFei Ding

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