Comparative proteomic analysis of human pancreatic juice: methodological study

Proteomics
Lu ZhouXingHua Lu

Abstract

Pancreatic cancer is the most lethal of all the common malignancies. Markers for early detection of this disease are urgently needed. Here, we optimized and applied a proteome analysis of human pancreatic juice to identify biomarkers for pancreatic cancer. Pancreatic juice samples, devoid of blood or bile contamination, were collected from patients with pancreatic cancer (n = 5), benign pancreatic diseases (n = 6), or cholelithiasis (n = 3) during endoscopic retrograde cholangiopancreatography (ERCP). After ultramembrane centrifugation sample preparation, pancreatic juice proteins were separated by 2-DE and identified by MALDI-TOF-MS. A 2-DE dataset of pancreatic juice from patients with cholelithiasis was established, consisting of 76 protein spots representing 22 different proteins. Disease-associated obstruction of the pancreatic duct strongly effected the protein composition of pancreatic juice. Concurrently, pancreatic juice from patients with pancreatic cancer was compared to nonmalignant controls with comparable obstruction of pancreatic ducts. Seven protein spots were identified that consistently appeared at changed levels in pancreatic juice from patients with pancreatic cancer. In conclusion, comparative proteomic ana...Continue Reading

References

Sep 1, 1982·Gut·P G Lankisch
Jan 20, 1999·Digestion·M LöhrG Klöppel
Oct 2, 2001·CA: a Cancer Journal for Clinicians·R T GreenleeM Thun
May 1, 2002·Electrophoresis·Xianglin YuanDominic M Desiderio
Jun 18, 2002·Hematology/oncology Clinics of North America·Christophe Rosty, Michael Goggins
Feb 5, 2004·Electrophoresis·Liping HuJie Chen
Apr 16, 2004·Molecular & Cellular Proteomics : MCP·Troels Zakarias KristiansenAkhilesh Pandey
Apr 20, 2004·Electrophoresis·Jae-Won YangGert Lubec
Aug 10, 2004·Electrophoresis·Elena ChernokalskayaJack T Leonard
Oct 12, 2004·Journal of Proteome Research·Mads GrønborgAkhilesh Pandey
Feb 3, 2005·Molecular & Cellular Proteomics : MCP·Ru ChenRuedi Aebersold

❮ Previous
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Citations

Dec 24, 2008·Digestive Diseases and Sciences·Ying LinRobert R Schade
Aug 21, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Umashankar K Ballehaninna, Ronald S Chamberlain
Nov 19, 2009·Expert Reviews in Molecular Medicine·Sreenivasa Chandana, Daruka Mahadevan
Jun 10, 2011·Molecular & Cellular Proteomics : MCP·Shalini MakawitaEleftherios P Diamandis
Dec 2, 2011·Pancreas·Courtney J DoyleC Max Schmidt
Jul 16, 2013·Pancreas·John A Williams
Nov 1, 2013·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Yantian FangChangyi Chen
Dec 16, 2011·World Journal of Gastroenterology : WJG·Chen SunRoland Andersson
Nov 5, 2013·Biochimica Et Biophysica Acta·Annarita Farina
Sep 17, 2009·Digestive Diseases and Sciences·Kathryn M DalbecNicholas J Zyromski
Jun 22, 2011·Journal of Cancer Research and Clinical Oncology·Jeong Youp ParkSi Young Song
Oct 27, 2010·Expert Review of Proteomics·María Gómez-LázaroMichael Schrader
Nov 13, 2008·Expert Review of Molecular Diagnostics·Ulrike HaugHermann Brenner
Oct 30, 2012·Journal of Insect Physiology·Dominique ColinetMarylène Poirié
Oct 5, 2010·Biochimica Et Biophysica Acta·Subhankar ChakrabortySurinder K Batra
Jul 1, 2008·Proteomics. Clinical Applications·Mark E WeeksTatjana Crnogorac-Jurcevic
Jan 14, 2011·Proteomics·Daniela CecconiMassimo Donadelli
Mar 2, 2011·Proteomics. Clinical Applications·Joao A PauloDarwin L Conwell
Mar 18, 2015·Proteomics·Sheng PanRu Chen
Apr 16, 2015·Pancreas·Giovanni MarchegianiCarlos Fernández-Del Castillo
Jan 1, 2010·Cancers·Patrick MichlMalte Buchholz
Aug 19, 2014·World Journal of Gastrointestinal Endoscopy·Tomoyuki IwataHitoshi Yoshida
Feb 17, 2019·Cell Biology and Toxicology·Daniel AnsariRoland Andersson
Mar 6, 2009·Archives of Pathology & Laboratory Medicine·Prathibha RanganathanAkhilesh Pandey
Oct 30, 2010·Journal of Proteome Research·Pang-ning TengThomas P Conrads

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