Comparative proteomic analysis of Listeria monocytogenes ATCC 7644 exposed to a sublethal concentration of nisin

Journal of Proteomics
Kendi Nishino MiyamotoAdriano Brandelli

Abstract

Listeria monocytogenes infections have been frequently reported in many food poisoning outbreaks around the world. In this work, the protein repertoires of L. monocytogenes ATCC 7644 cells treated or not with a 10(-3)mg/mL nisin sublethal concentration, established by antimicrobial susceptibility tests, were analyzed by LC-MS/MS. Overall, 179 proteins were identified, 9 of them more abundant in nisin-treated samples, and 4 more abundant in non-treated control samples. In nisin treated cells, proteins associated to oxidative stress response showed higher abundance. Also, the higher abundance of an enzyme related to the production of cell membrane lipids upon nisin exposure is suggestive of both a failure in conventional cell division mechanism and the activation of an alternative L-form mediated division mechanism. Finally, flagellar and motility proteins' overexpression upon nisin exposure is indicative of increased bacterial motility in response to the bacteriocin. Taken together, these results provide new insights on nisin effects on L. monocytogenes cells and on how this bacterium may overcome a bacteriocin-containing environment. The antimicrobial mechanism of nisin on target bacterial cells has been extensively studied sin...Continue Reading

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Citations

Apr 26, 2016·Journal of Proteomics·Tamara MartinovićDjuro Josić
Sep 22, 2016·Expert Review of Proteomics·Osmel Fleitas MartinezOctávio Luiz Franco
Sep 9, 2020·Scientific Reports·K A YamamotoM R Soares
Jun 27, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Fernanda Cristina Bergamo AlvesAry Fernandes Junior
Jun 11, 2019·Frontiers in Microbiology·Alexey S Vasilchenko, Eugene A Rogozhin
Mar 27, 2021·International Journal of Food Microbiology·Cristian Mauricio Barreto PinillaAdriano Brandelli
May 11, 2021·Reports of Biochemistry & Molecular Biology·Noha El-Sayed IbrahimMarwa Abdelgwad
Feb 25, 2020·Journal of Proteome Research·Adithi R VaradarajanBernd Wollscheid
Sep 24, 2021·Applied Microbiology and Biotechnology·Natan de Jesus Pimentel-FilhoMaria Cristina Dantas Vanetti

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