Comparative proteomic analysis of Litopenaeus vannamei gills after vaccination with two WSSV structural proteins

Fish & Shellfish Immunology
Li-Hao ChenJyh-Ming Tsai

Abstract

White spot syndrome virus (WSSV) is one of the most devastating viral pathogens of cultured shrimp worldwide. Recently published papers show the ability of WSSV structural protein VP28 to vaccinate shrimp and raise protection against the virus. This study attempted to identify the joining proteins of the aforementioned shrimp quasi-immune response by proteomic analysis. The other envelope protein, VP36B, was used as the non-protective subunit vaccine control. Shrimp were intramuscularly injected with rVPs or PBS on day 1 and day 4 and then on day 7 their gill tissues were sampled. The two-dimensional electrophoresis (2-DE) patterns of gill proteins between vaccinated and PBS groups were compared and 20 differentially expressed proteins identified by mass spectrometry, some of which were validated in gill and hemocyte tissues using real-time quantitative RT-PCR. Many of identified proteins and their expression levels also linked with the shrimp response during WSSV infection. The list of up-regulated protein spots found exclusively in rVP28-vaccinated shrimp include calreticulin and heat shock protein 70 with chaperone properties, ubiquitin, and others. The two serine proteases, chymotrypsin and trypsin, were significantly incre...Continue Reading

References

Oct 7, 2000·Diseases of Aquatic Organisms·C A VenegasK Murog
Apr 18, 2001·Trends in Cell Biology·S JohnsonP Eggleton
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 21, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anne-Estelle KiefferMarie-Héléne Metz-Boutigue
Aug 21, 2003·Lancet·A Graham Pockley
Apr 28, 2004·Fish & Shellfish Immunology·Jeroen WitteveldtMariëlle C W van Hulten
Aug 17, 2005·Insect Biochemistry and Molecular Biology·Salvador HerreroJules Beekwilder
Dec 26, 2006·Developmental and Comparative Immunology·Hao-Ching WangChu-Fang Lo
Mar 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sylvie AlonsoGeorgiana E Purdy
Apr 24, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Wei LuanaJianhai Xiang
May 15, 2007·FEBS Letters·Stuart K CalderwoodJimmy R Theriault
Mar 10, 2009·Fish & Shellfish Immunology·Fei ZhuZi-Rong Xu
Aug 6, 2010·Journal of Virology·Apiruck WatthanasurorotKenneth Söderhäll
Sep 13, 2011·Developmental and Comparative Immunology·Yi MuJin-Xing Wang
Mar 21, 2012·Journal of Invertebrate Pathology·Timothy W Flegel
Mar 22, 2012·Journal of Invertebrate Pathology·D V LightnerS A Navarro
Mar 22, 2012·Journal of Invertebrate Pathology·Shaun M MossJeffrey M Lotz
Oct 13, 2012·Fish & Shellfish Immunology·Anchalee TassanakajonSureerat Tang
Feb 5, 2013·Journal of Molecular Cell Biology·Apiruck WatthanasurorotIrene Söderhäll
May 2, 2014·Developmental and Comparative Immunology·Syed Khader Syed Musthaq, Jimmy Kwang

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Citations

May 20, 2017·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Thorben GrauGerrit Joop
Mar 14, 2019·Frontiers in Physiology·Tong HaoJinsheng Sun

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