Comparative proteomic analysis of Mycobacterium tuberculosis strain H37Rv versus H37Ra

International Journal of Mycobacteriology
Lingaraja JenaBhaskar Chinnaiah Harinath

Abstract

Mycobacterium tuberculosis (MTB) H37Ra is an attenuated tubercle bacillus closely related to the virulent type strain MTB H37Rv. In spite of extensive study, variation in virulence between the MTB H37Rv and MTB H37Ra strains is still to be understood. The difference in protein expression or structure due to mutation may probably be an important factor for the virulence property of MTB H37Rv strain. In this study, a whole proteome comparison between these two strains was carried out using bioinformatics approaches to elucidate differences in their protein sequences. On comparison of whole proteome using NCBI standalone BLAST program between these two strains, 3759 identical proteins in both the strains out of 4003 proteins were revealed in MTB H37Rv and 4034 proteins were revealed in MTB H37Ra; 244 proteins of MTB H37Rv and 260 proteins of MTB H37Ra were found to be non-identical. A total of 172 proteins were identified with mutations (Insertions/deletions/substitutions) in MTB H37Ra while 53 proteins of MTB H37Rv and 85 proteins of MTB H37Ra were found to be distinct. Among 244 non-identical proteins, 19 proteins were reported to have an important biological function; In this study, mutation was shown in these proteins of MTB H...Continue Reading

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Citations

Oct 7, 2008·Trends in Microbiology·Michelle RyndakIssar Smith
Jul 5, 2018·Frontiers in Microbiology·Sneha M PintoT S Keshava Prasad
Dec 14, 2018·Molecular Diversity·Kristina PavićBranka Zorc
Apr 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Weronika AmbrożkiewiczJan Zitko
Nov 6, 2020·Journal of Zhejiang University. Science. B·Elba Rodríguez-HernándezFeliciano Milián-Suazo
Oct 30, 2020·Scientific Reports·Diane Frances LeeMark Andrew Chambers

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