Comparative Proteomic and Morphological Change Analyses of Staphylococcus aureus During Resuscitation From Prolonged Freezing

Frontiers in Microbiology
Biao SuoZhilu Ai

Abstract

When frozen, Staphylococcus aureus survives in a sublethally injured state. However, S. aureus can recover at a suitable temperature, which poses a threat to food safety. To elucidate the resuscitation mechanism of freezing survived S. aureus, we used cells stored at -18°C for 90 days as controls. After resuscitating the survived cells at 37°C, the viable cell numbers were determined on tryptic soy agar with 0.6% yeast extract (TSAYE), and the non-injured-cell numbers were determined on TSAYE supplemented with 10% NaCl. The results showed that the total viable cell number did not increase within the first 3 h of resuscitation, but the osmotic regulation ability of freezing survived cells gradually recovered to the level of healthy cells, which was evidenced by the lack of difference between the two samples seen by differential cell enumeration. Scanning electron microscopy (SEM) showed that, compared to late exponential stage cells, some frozen survived cells underwent splitting and cell lysis due to deep distortion and membrane rupture. Transmission electron microscopy (TEM) showed that, in most of the frozen survived cells, the nucleoids (low electronic density area) were loose, and the cytoplasmic matrices (high electronic d...Continue Reading

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Citations

Aug 11, 2019·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·Yuexia WangBiao Suo
Apr 17, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Gerson-Dirceu LópezChiara Carazzone
Feb 6, 2021·Journal of Applied Microbiology·D Panisello YagüeT Pearson

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Datasets Mentioned

BETA
IPX0001087000

Methods Mentioned

BETA
environmental stress
scanning electron microscopy
transmission electron microscopy
Assay
phosphotransferase

Software Mentioned

Proteomics
Peaks Studio
Search Tool of the Retrieval of
Cytoscape
STRING
BLAST2GO
Progenesis QI

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