Comparative proteomic network signatures in seminal plasma of infertile men as a function of reactive oxygen species

Clinical Proteomics
Ashok AgarwalEdmund Sabanegh

Abstract

Reactive oxygen species (ROS) plays a major role in the pathology of male infertility. It is an independent biomarker of sperm function. Seminal plasma is a natural reservoir of antioxidants responsible for the nourishment, protection, capacitation, and motility of sperm within the female reproductive tract resulting in successful fertilization and implantation of the embryo. A comparative proteomic analysis of seminal plasma proteins from fertile men and infertile men with varying levels of ROS was carried out to identify signature proteins involved in ROS-mediated reproductive dysfunction. A total of 42 infertile men presenting with infertility and 17 proven fertile donors were enrolled in the study. ROS levels were measured in the seminal ejaculates by chemiluminescence assay. Infertile men were subdivided into Low ROS (0-<93 RLU/s/10(6) sperm; n = 11), Medium ROS (>93-500 RLU/s/10(6) sperm; n = 17) and High ROS (>500 RLU/s/10(6) sperm; n = 14) groups and compared with fertile men (4-50 RLU/s/10(6) sperm). 4 subjects from fertile group and 4 each from the Low, Medium and High ROS were pooled. 1D gel electrophoresis followed by in-gel digestion and LC/MS-MS in a LTQ-Orbitrap Elite hybrid mass spectrometer system was used for ...Continue Reading

References

Sep 1, 1992·American Journal of Reproductive Immunology : AJRI·G SkibinskiK James
Nov 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P Bornstein
Jan 1, 1987·Advances in Experimental Medicine and Biology·D J MillerR L Ax
Jan 1, 1994·Reproduction, Fertility, and Development·R J Aitken
Dec 1, 1996·Urology·R K Sharma, A Agarwal
Feb 1, 1997·International Journal of Andrology·G WennemuthG Aumüller
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·R L RobkerJ S Richards
Jun 7, 2000·Urology·F F PasqualottoA Agarwal
Feb 12, 2002·Peptides·David FernándezLuis Casis
Jun 19, 2002·American Journal of Reproductive Immunology : AJRI·Adil A BabikerBo Nilsson
Oct 19, 2002·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Hitoshi YokoyamaKouji Matsushima
May 17, 2003·Fertility and Sterility·Ashok AgarwalMohamed A Bedaiwy
Aug 9, 2003·The Journal of Biological Chemistry·Darryl L RussellJoAnne S Richards
Nov 11, 2003·Fertility and Sterility·Carlo A PalmeriniGiuseppe Arienti
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Mar 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Francois BlondeauPeter S McPherson
Apr 28, 2004·Molecular and Cellular Endocrinology·P VernetJ R Drevet
Jul 30, 2004·Molecular and Cellular Biology·David W PowellAndrew J Link
Aug 5, 2004·Journal of Andrology·Jon IrazustaLuis Casis
May 17, 2005·Blood Cells, Molecules & Diseases·Robert SullivanGilles Frenette
May 24, 2005·Cell and Tissue Research·Sarah A Robertson
Jan 31, 2006·Molecular and Cellular Endocrinology·Wai-Sum OP H Chow
May 20, 2006·Genome Biology·Bartosz Pilch, Matthias Mann
Sep 2, 2006·Journal of Proteome Research·Boris ZybailovMichael P Washburn
Oct 10, 2006·Fertility and Sterility·Ashok AgarwalSuresh C Sikka
Apr 18, 2007·Human Reproduction·C G RavinaM Luz Candenas
Oct 24, 2007·Fertility and Sterility·Nerea SubiránJon Irazusta
Feb 19, 2008·Human Reproduction Update·Kelton Tremellen
Jun 19, 2008·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Fnu DeepinderAshok Agarwal
Jul 30, 2008·Cell Stress & Chaperones·Harm H KampingaLawrence E Hightower

❮ Previous
Next ❯

Citations

Jun 5, 2016·Fertility and Sterility·Ashok AgarwalEdmund Sabanegh
Nov 19, 2017·Scientific Reports·Seyed Morteza RazaviAli Salehzadeh-Yazdi
Mar 8, 2019·Reproduction : the Official Journal of the Society for the Study of Fertility·T LeahyS P de Graaf
May 1, 2018·Reproductive Biology and Endocrinology : RB&E·Luna SamantaAshok Agarwal
Aug 20, 2020·Andrologia·Kathy A RobertAshok Agarwal
Feb 16, 2019·International Journal of Molecular Sciences·Manesh Kumar Panner SelvamPeter N Pushparaj
Sep 30, 2020·International Journal of Molecular Sciences·Luz Candenas, Rosanna Chianese
Jan 17, 2019·International Journal of Molecular Sciences·Manesh Kumar Panner SelvamLuna Samanta
Jul 12, 2020·International Journal of Molecular Sciences·Manesh Kumar Panner SelvamAshok Agarwal
Jul 28, 2020·International Journal of Molecular Sciences·Alba Fernandez-EncinasJordi Benet
Dec 4, 2020·International Journal of Molecular Sciences·Rossella CannarellaSandro La Vignera
Nov 18, 2020·International Journal of Molecular Sciences·Fengli WangShuiqiao Yuan
Feb 28, 2021·Molecular and Cellular Endocrinology·Rashid A Aldahhan, Peter G Stanton
Nov 25, 2021·Advanced Biology·Tricia Rowlison, Pierre Comizzoli

❮ Previous
Next ❯

Methods Mentioned

BETA
protein folding
acetylation
ubiquitination

Software Mentioned

Mascot
Scaffold
Proteome
! Tandem
CYCLONE
STRAP
Software Tools for Researching Annotations of Proteins ( STRAP )
IPA ( Ingenuity Pathway Analysis ) Systems
IPA
Sequest

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.