Comparative Proteomics Analyses of Two Races of Fusarium oxysporum f. sp. conglutinans that Differ in Pathogenicity

Scientific Reports
Erfeng LiBingyan Xie

Abstract

Fusarium oxysporum is a soil-inhabiting fungus that induces vascular wilt and root rot in a variety of plants. F. oxysporum f. sp. conglutinans (Foc), which comprises two races, can cause wilt disease in cabbage. Compared with race 1 (52557(-TM), R1), race 2 (58385(-TM), R2) exhibits much stronger pathogenicity. Here, we provide the first proteome reference maps for Foc mycelium and conidia and identify 145 proteins with different abundances among the two races. Of these proteins, most of the high-abundance proteins in the R2 mycelium and conidia are involved in carbohydrate, amino acid and ion metabolism, which indicates that these proteins may play important roles in isolate R2's stronger pathogenicity. The expression levels of 20 typical genes demonstrate similarly altered patterns compared to the proteomic analysis. The protein glucanosyltransferase, which is involved in carbohydrate metabolism, was selected for research. We knocked out the corresponding gene (gas1) and found that Foc-∆gas1 significantly reduced growth rate and virulence compared with wild type isolates. These results deepened our understanding of the proteins related to F. oxysporum pathogenicity in cabbage Fusarium wilt and provided new opportunities to c...Continue Reading

References

Sep 1, 1992·Immunology Today·R Matthews, J Burnie
Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg
Oct 1, 1996·The Plant Cell·K E Hammond-Kosack, J D Jones
Mar 15, 1997·The Biochemical Journal·P Wojtaszek
Jun 24, 2000·Nature·A Pandey, M Mann
Aug 11, 2001·Cellular and Molecular Life Sciences : CMLS·V Pancholi
May 20, 2003·Current Opinion in Plant Biology·Nicholas J Kruger, Antje von Schaewen
Dec 20, 2003·Current Genetics·Jasmine GrinyerBen R Herbert
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon
Oct 7, 2004·Fungal Genetics and Biology : FG & B·Jae-Hyuk YuClaudio Scazzocchio
Dec 16, 2005·Molecular Plant-microbe Interactions : MPMI·Zaira CaracuelM Isabel G Roncero
Feb 25, 2006·Fungal Genetics and Biology : FG & B·J FuB L Wickes
Mar 18, 2006·Proteomics·Francisco Javier Fernández-AceroJesús Jorrín
Oct 20, 2006·Proteomics·William Yajima, Nat N V Kav
Aug 8, 2007·Trends in Biotechnology·Yonghyun KimMark R Marten
Aug 24, 2007·FEMS Yeast Research·Luiz Augusto PereiraCélia Maria de Almeida Soares
Apr 25, 2008·Current Protocols in Immunology·Zihai Li, Pramod Srivastava
Jun 27, 2008·BMC Genomics·Wenchuan LengQi Jin
Feb 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Leah E CowenSusan Lindquist
Feb 25, 2009·Molecular Plant Pathology·Sandra NoirRalph Panstruga
Mar 17, 2009·Current Microbiology·Lvgao QinKeping Chen
Apr 30, 2009·Molecular Plant Pathology·Caroline B Michielse, Martijn Rep
Sep 29, 2011·Molecular Plant-microbe Interactions : MPMI·Louise F ThatcherJohn M Manners

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Citations

Mar 28, 2016·The Protein Journal·Jameel R Al-ObaidiAbu Seman Idris
Mar 11, 2017·Preparative Biochemistry & Biotechnology·Nathália Gonsales da Rosa-GarzonHamilton Cabral
Jul 4, 2019·World Journal of Microbiology & Biotechnology·Yamily Y Burgos-CanulIgnacio Islas-Flores
May 11, 2017·Annual Review of Phytopathology·Günter BraderAngela Sessitsch
Sep 26, 2020·Frontiers in Plant Science·Silvia Rodríguez-PiresEduardo A Espeso

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Methods Mentioned

BETA
environmental stress
PCR
electrophoresis
phosphotransferase

Software Mentioned

MS BioTool
SAS
MASCOT
COG
TargetP
BLAST
flexAnalysis
DeCyder

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