Comparative proteomics of a tor inducible Aspergillus fumigatus mutant reveals involvement of the Tor kinase in iron regulation

Proteomics
Clara BaldinAxel A Brakhage

Abstract

The Tor (target of rapamycin) kinase is one of the major regulatory nodes in eukaryotes. Here, we analyzed the Tor kinase in Aspergillus fumigatus, which is the most important airborne fungal pathogen of humans. Because deletion of the single tor gene was apparently lethal, we generated a conditional lethal tor mutant by replacing the endogenous tor gene by the inducible xylp-tor gene cassette. By both 2DE and gel-free LC-MS/MS, we found that Tor controls a variety of proteins involved in nutrient sensing, stress response, cell cycle progression, protein biosynthesis and degradation, but also processes in mitochondria, such as respiration and ornithine metabolism, which is required for siderophore formation. qRT-PCR analyses indicated that mRNA levels of ornithine biosynthesis genes were increased under iron limitation. When tor was repressed, iron regulation was lost. In a deletion mutant of the iron regulator HapX also carrying the xylp-tor cassette, the regulation upon iron deprivation was similar to that of the single tor inducible mutant strain. In line, hapX expression was significantly reduced when tor was repressed. Thus, Tor acts either upstream of HapX or independently of HapX as a repressor of the ornithine biosynthe...Continue Reading

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Citations

Aug 19, 2015·Applied Microbiology and Biotechnology·Derek J MatternAxel A Brakhage
Jul 5, 2016·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Muyan ChenTianming Wang
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Dec 24, 2019·Frontiers in Microbiology·Ingo BauerHubertus Haas
Jan 30, 2019·Frontiers in Microbiology·João Henrique Tadini Marilhano FabriIran Malavazi
Feb 26, 2019·Frontiers in Pharmacology·Tomas Di MambroEmanuele Marra
Oct 11, 2020·Research in Microbiology·Ján Víglaš, Petra Olejníková
Nov 17, 2020·Frontiers in Molecular Biosciences·Sandeep VellankiSoo Chan Lee
Apr 27, 2021·Frontiers in Microbiology·João Henrique Tadini Marilhano FabriIran Malavazi
Jul 15, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·María Teresa Martínez-Pastor, Sergi Puig

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