Comparative proteomics of human embryonic stem cells and embryonal carcinoma cells.

Proteomics
Raghothama ChaerkadyAkhilesh Pandey

Abstract

Pluripotent human embryonic stem cells (ESCs) can be differentiated in vitro into a variety of cells which hold promise for transplantation therapy. Human embryonal carcinoma cells (ECCs), stem cells of human teratocarcinomas, are considered a close but malignant counterpart to human ESCs. In this study, a comprehensive quantitative proteomic analysis of ESCs and ECCs was carried out using the iTRAQ method. Using two-dimensional LC and MS/MS analyses, we identified and quantitated approximately 1800 proteins. Among these are proteins associated with pluripotency and development as well as tight junction signaling and TGFbeta receptor pathway. Nearly approximately 200 proteins exhibit more than twofold difference in abundance between ESCs and ECCs. Examples of early developmental markers high in ESCs include beta-galactoside-binding lectin, undifferentiated embryonic cell transcription factor-1, DNA cytosine methyltransferase 3beta isoform-B, melanoma antigen family-A4, and interferon-induced transmembrane protein-1. In contrast, CD99-antigen (CD99), growth differentiation factor-3, cellular retinoic acid binding protein-2, and developmental pluripotency associated-4 were among the highly expressed proteins in ECCs. Several prot...Continue Reading

References

Dec 5, 1998·Science·J Gearhart
Apr 25, 2002·Molecular and Cellular Biology·Hugh D CampbellKlaus I Matthaei
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jamie M SpergerJames A Thomson
Aug 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Robert H BlellochRudolf Jaenisch
Dec 30, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christina E Hoei-HansenEwa Rajpert-De Meyts
Apr 8, 2006·Molecular & Cellular Proteomics : MCP·Dennis Van HoofJeroen Krijgsveld
May 3, 2006·Cancer Research·Tobias PeikertSuzy A A Comhair
Sep 23, 2006·Stem Cells·Joanna Maldonado-SaldiviaPatrick S Western
Jun 8, 2007·Nature·Keisuke OkitaShinya Yamanaka
Jul 3, 2007·Nature·Tarjei S MikkelsenBradley E Bernstein
Jul 20, 2007·Nature·Janet Rossant
Aug 10, 2007·British Journal of Haematology·Christian FlothoCharlotte M Niemeyer
Aug 29, 2007·Nature Biotechnology·Alexander MeissnerRudolf Jaenisch
Sep 28, 2007·Nature·Monya Baker
Oct 31, 2007·Molecular and Cellular Biology·Kevin Myant, Irina Stancheva
Nov 24, 2007·Journal of Proteome Research·Jermel WatkinsDaniel F Bogenhagen
Jan 17, 2008·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Jutta RiesEmeka Nkenke
May 21, 2008·Journal of Proteome Research·Wilma DormeyerJeroen Krijgsveld
Jun 3, 2008·Progress in Neurobiology·Koichi HaraCesar V Borlongan
Jun 17, 2008·Biochemical and Biophysical Research Communications·Moon-Chang ChoiYung-Jue Bang
Jul 5, 2008·Cancer Cell·Francesca BernassolaGerry Melino
Oct 25, 2008·Nucleic Acids Research·Kumaran KandasamyAkhilesh Pandey
Nov 8, 2008·Nucleic Acids Research·T S Keshava PrasadAkhilesh Pandey
Jan 29, 2009·Journal of Proteome Research·Raghothama ChaerkadyAkhilesh Pandey

❮ Previous
Next ❯

Citations

Jul 20, 2012·Expert Review of Proteomics·Onno KranenburgConnie R Jimenez
Sep 5, 2015·International Journal of Molecular Sciences·Chenxia Hu, Lanjuan Li
Jan 13, 2011·Proteomics·Chris S HughesGilles A Lajoie
Jul 22, 2014·Journal of Tissue Engineering and Regenerative Medicine·Akram MansouriEsmaeil Ebrahimie
Oct 1, 2011·Proteomics. Clinical Applications·Roberto ScatenaBruno Giardina
Feb 28, 2015·Current Pathobiology Reports·Laura E McNamaraKarl V Burgess
Jul 30, 2010·Stem Cell Reviews and Reports·Maria P De MiguelYago Alcaina
Mar 23, 2019·Genes & Cancer·Candace L KerrVenu Raman
Jul 1, 2020·International Journal of Molecular Sciences·Finn Edler von Eyben, Jorge Parraga-Alava
Dec 12, 2012·Human Reproduction Update·H T NguyenC Spits
Aug 12, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Daniel NettersheimHubert Schorle
Jul 1, 2017·Proteomics·Elisabeth GovaertDieter Deforce

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Jamie M SpergerJames A Thomson
Molecular & Cellular Proteomics : MCP
Dennis van HoofJeroen Krijgsveld
Nature Reviews. Cancer
Ricardo PardalSean J Morrison
© 2021 Meta ULC. All rights reserved