Comparative proteomics of pulmonary tumors with neuroendocrine differentiation

Journal of Proteome Research
Nam Hoon ChoDong Su Kim

Abstract

We aimed to evaluate neuroendocrine pulmonary tumors (NEPT) by a novel method involving map tree construction by comparing all of the protein spots. We performed a proteomics analysis to assess the similarities in protein expression between neuroendocrine pulmonary tumors (NEPT), including typical carcinoids (TC), atypical carcinoids (AC), large cell neuroendocrine carcinomas (LCNEC) and small cell carcinomas (SCLC). Total protein lysates were obtained from seven histologically confirmed frozen NEPT tissues, including 1TC, 2 SCLC, and 4 cases ranging from AC to LCNEC. 2-DE demonstrated that TC was similar to normal lung. AC, LCNEC, and SCLC were similar to each other, forming a group separate from TC, however, SCLC at an early stage showed a similarity to TC. MALDI analysis detected 9 surrogate endpoint biomarkers, including eIF5A1, GST M3, cytokeratin 18 (CK 18), FK506-binding protein p59, p63, MAGE-D2, mitochondrial short-chain enoyl-coenzyme A hydratase 1, tranferrin and poly (rC) binding protein 1. Immunohistochemical staining revealed a gradual decrease in expression rate of p63 and CK 18 with poor differentiation of NEPT. Our results demonstrate that (1) the comparative proteomics of NEPT match the WHO classification exce...Continue Reading

References

Nov 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·A BhattacharjeeM Meyerson
Apr 16, 2002·Science·Wolfgang EnardSvante Pääbo
Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Carl VirtanenMichael H Jones
Oct 9, 2002·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Yoko FukunagaToshihiko Ishida
Jun 5, 2003·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Tatyana A ZhukovMelvyn S Tockman
Aug 21, 2003·Lancet·Kiyoshi YanagisawaDavid P Carbone
Dec 6, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Shi-Xu JiangIsao Okayasu

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Citations

Sep 27, 2006·Pituitary·Eva Hofsli
Oct 1, 2007·Expert Review of Respiratory Medicine·Stewart J Levine
Dec 27, 2015·Biochimica Et Biophysica Acta·Céline PirlotYvette Habraken
Apr 13, 2012·Current Problems in Cancer·Maria Catherine Pietanza, Charles M Rudin
Feb 1, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jun ZhangJianwu Tang
Feb 1, 2008·Proteomics. Clinical Applications·Franck BrouillardMario Ollero
Jun 22, 2011·Nature Reviews. Clinical Oncology·William N William, Bonnie S Glisson
Apr 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Laleh KamalianYouqiang Ke

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