Comparative proteomics of respiratory exosomes in cystic fibrosis, primary ciliary dyskinesia and asthma

Journal of Proteomics
Virginie Rollet-CohenIda Chiara Guerrera

Abstract

Cystic fibrosis (CF) and primary ciliary dyskinesia (PCD) are pulmonary genetic disorders associated with inflammation and heterogeneous progression of the lung disease. We hypothesized that respiratory exosomes, nanovesicles circulating in the respiratory tract, may be involved in the progression of inflammation-related lung damage. We compared proteomic content of respiratory exosomes isolated from bronchoalveolar lavage fluid in CF and PCD to asthma (A), a condition also associated with inflammation but with less severe lung damage. BALF were obtained from 3 CF, 3 PCD and 6 A patients. Exosomes were isolated from BALF by ultracentrifugations and characterized using immunoelectron microscopy and western-blot. Exosomal protein analysis was performed by high-resolution mass spectrometry using label-free quantification. Exosome enrichment was validated by electron microscopy and immunodetection of CD9, CD63 and ALIX. Mass spectrometry analysis allowed the quantification of 665 proteins, of which 14 were statistically differential according to the disease. PCD and CF exosomes contained higher levels of antioxidant proteins (Superoxide-dismutase, Glutathione peroxidase-3, Peroxiredoxin-5) and proteins involved in leukocyte chemota...Continue Reading

Citations

Jan 12, 2020·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Phoomphut SangaphunchaiLucy C Fairclough
Feb 26, 2019·Frontiers in Pediatrics·Natalia Hernandez-PachecoCarlos Flores
Sep 16, 2020·International Journal of Molecular Sciences·Sebastien GauthierIsabelle Sermet-Gaudelus
Jan 11, 2020·Frontiers in Endocrinology·Yi LiLicheng Zhang
Nov 17, 2020·European Journal of Medicinal Chemistry·Margarida D Amaral
Jan 23, 2021·International Journal of Molecular Sciences·José A CañasVictoria del Pozo
Jan 26, 2021·Molecular Biotechnology·Talitha Keren KurianNirmal Mazumder
Mar 14, 2021·Thorax·Anne TrappeJudith A Coppinger
May 1, 2021·International Journal of Molecular Sciences·Lukasz ZarebaMalgorzata Czystowska-Kuzmicz
Jun 3, 2021·International Journal of Molecular Sciences·Bilal Alashkar AlhamweHolger Garn
Aug 14, 2021·Journal of Cellular Physiology·Ziyu LiuYing Zhang

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