Comparative proteomics of two Mycoplasma hyopneumoniae strains and Mycoplasma flocculare identified potential porcine enzootic pneumonia determinants

Virulence
Jéssica A PaesHenrique B Ferreira

Abstract

Mycoplasma hyopneumoniae and Mycoplasma flocculare are genetically similar bacteria, which coinhabit the porcine respiratory tract. These mycoplasmas share most of the known virulence factors, but, while M. hyopneumoniae causes porcine enzootic pneumonia (PEP), M. flocculare is a commensal species. To identify potential PEP determinants and provide novel insights on mycoplasma-host interactions, the whole cell proteomes of two M. hyopneumoniae strains, one pathogenic (7448) and other non-pathogenic (J), and M. flocculare were compared. A cell fractioning approach combined with mass spectrometry (LC-MS/MS) proteomics was used to analyze cytoplasmic and surface-enriched protein fractions. Average detection of ~ 50% of the predicted proteomes of M. hyopneumoniae 7448 and J, and M. flocculare was achieved. Many of the identified proteins were differentially represented in M. hyopneumoniae 7448 in comparison to M. hyopneumoniae J and M. flocculare, including potential PEP determinants, such as adhesins, proteases, and redox-balancing proteins, among others. The LC-MS/MS data also provided experimental validation for several genes previously regarded as hypothetical for all analyzed mycoplasmas, including some coding for proteins bea...Continue Reading

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Citations

Apr 7, 2018·Molecular Microbiology·Mariana Galvao FerrariniMarie-France Sagot
Oct 12, 2019·Scientific Reports·Veronica Maria JarockiSteven Philip Djordjevic
Dec 9, 2020·Virulence·Fernanda M A Leal ZimmerHenrique Bunselmeyer Ferreira
Jan 4, 2020·Microbial Pathogenesis·Lais Del Prá Netto MachadoHenrique Bunselmeyer Ferreira
Oct 6, 2019·Veterinary Microbiology·Sarah FourourCorinne Marois-Créhan
May 10, 2021·Veterinary Research·Dominiek MaesFreddy Haesebrouck

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Methods Mentioned

BETA
Protein Assay
acetylation

Software Mentioned

BLAST2GO
PrediSi
SecretomeP
Mascot Generic Format
Phobius
PRED
TM
Mascot Search Engine
BlastP
msConvert

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