Comparative reactivity of the myeloperoxidase-derived oxidants HOCl and HOSCN with low-density lipoprotein (LDL): Implications for foam cell formation in atherosclerosis

Archives of Biochemistry and Biophysics
Fahd O IsmaelClare L Hawkins

Abstract

Atherosclerosis is characterised by the accumulation of lipids within macrophages in the artery wall. Low-density lipoprotein (LDL) is the source of this lipid, owing to the uptake of oxidised LDL by scavenger receptors. Myeloperoxidase (MPO) released by leukocytes during inflammation produces oxidants that are implicated in atherosclerosis. Modification of LDL by the MPO oxidant hypochlorous acid (HOCl), results in extensive lipid accumulation by macrophages. However, the reactivity of the other major MPO oxidant, hypothiocyanous acid (HOSCN) with LDL is poorly characterised, which is significant given that thiocyanate is the favoured substrate for MPO. In this study, we comprehensively compare the reactivity of HOCl and HOSCN with LDL, and show key differences in the profile of oxidative damage observed. HOSCN selectively modifies Cys residues on apolipoprotein B100, and oxidises cholesteryl esters resulting in formation of lipid hydroperoxides, 9-hydroxy-10,12-octadecadienoic acid (9-HODE) and F2-isoprostanes. The modification of LDL by HOSCN results macrophage lipid accumulation, though generally to a lesser extent than HOCl-modified LDL. This suggests that a change in the ratio of HOSCN:HOCl formation by MPO from variation...Continue Reading

References

Jan 1, 1987·International Archives of Occupational and Environmental Health·K Husgafvel-PursiainenP Einistö
Mar 12, 2008·Antioxidants & Redox Signaling·Michael J DaviesMartin D Rees
Nov 6, 2009·Antioxidants & Redox Signaling·Irena LevitanPapasani V Subbaiah
Feb 20, 2010·Chemistry and Physics of Lipids·Alexej V SokolovOleg M Panasenko
Mar 19, 2014·Chemistry and Physics of Lipids·Alexej V SokolovOleg M Panasenko

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Citations

Apr 30, 2016·Oxidative Medicine and Cellular Longevity·Bojana KisicLjiljana Popovic
Mar 31, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Thomas M JeitnerGraham R Moran
Sep 23, 2020·Nutrients·Tawar QaradakhiAnthony Zulli
Aug 23, 2020·International Journal of Molecular Sciences·Michele ProvenzanoMichele Andreucci
Dec 23, 2019·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Simon CorreaFinnian R Mc Causland
Jun 15, 2021·Frontiers in Cell and Developmental Biology·Divya Saro Varghese, Bassam R Ali
Jul 12, 2021·Free Radical Biology & Medicine·Clare L Hawkins, Michael J Davies

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