Comparative resistance of food proteins to adult and infant in vitro digestion models

Molecular Nutrition & Food Research
Didier DupontAlan R Mackie

Abstract

IgE-mediated allergy to milk and egg is widespread in industrialised countries and mainly affects infants and young children. It may be connected to an incomplete digestion of dietary proteins causing an inappropriate immune response in the gut. In order to study this, a biochemical model of infant gastroduodenal digestion has been developed, which has reduced levels of protease (eightfold for pepsin and tenfold for trypsin and chymotrypsin), phosphatidylcholine and bile salts, compared with the adult model. This model has been used to study the behaviour of three characterised food-relevant proteins (bovine beta-lactoglobulin (beta-Lg), beta-casein (beta-CN) and hen's egg ovalbumin), all of which are relevant cows' milk and hens' egg allergens. Digestion products were characterised using electrophoresis, immunochemical techniques and MS. These showed that ovalbumin and beta-CN were digested more slowly using the infant model compared with the adult conditions. Resistant fragments of beta-CN were found in the infant model, which correspond to previously identified IgE epitopes. Surprisingly, beta-Lg was more extensively degraded in the infant model compared with the adult one. This difference was attributed to the tenfold reduc...Continue Reading

References

Dec 25, 1983·Journal of Molecular Biology·R M Sweet, D Eisenberg
Oct 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D G SchmidtE C van Beresteijn
Oct 1, 1996·Nature Biotechnology·J D AstwoodR L Fuchs
May 18, 1999·The Journal of Allergy and Clinical Immunology·H A Sampson
Nov 4, 2000·Biochimica Et Biophysica Acta·R MäntyjärviT Virtanen
Apr 12, 2001·Allergy·J M Wal
Sep 1, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P ChatchateeH A Sampson
Aug 10, 2002·The Journal of Allergy and Clinical Immunology·Kirsi-Marjut JärvinenHugh A Sampson
Jun 27, 2003·Environmental Health Perspectives·Gary BannonDennis M Hinton
Nov 7, 2003·Archives of Disease in Childhood. Fetal and Neonatal Edition·T I Omari, G P Davidson
May 12, 2004·Biochimica Et Biophysica Acta·F Javier MorenoE N Clare Mills
Nov 16, 2004·Critical Reviews in Food Science and Nutrition·E N Clare MillsPeter R Shewry
Feb 8, 2005·The Journal of Allergy and Clinical Immunology·Eva UntersmayrErika Jensen-Jarolim
Apr 19, 2005·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Karen WalthallTracey Zoetis
Nov 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Isabella SchöllErika Jensen-Jarolim
Oct 4, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T EiweggerZ Szépfalusi
Jan 12, 2007·The Journal of Allergy and Clinical Immunology·Eva UntersmayrLars K Poulsen
Jan 24, 2007·Biochimica Et Biophysica Acta·Yoshinori Mine, Marie Yang
May 23, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R R CoccoH A Sampson
Jul 14, 2007·The Journal of Allergy and Clinical Immunology·Roberto J RonaCharlotte Madsen
Apr 18, 2008·Journal of Agricultural and Food Chemistry·Giuseppina MandalariMartin S J Wickham
Jun 10, 2008·The Journal of Allergy and Clinical Immunology·Eva Untersmayr, Erika Jensen-Jarolim
Nov 26, 2008·Molecular Nutrition & Food Research·Stefan Vieths, Karin Hoffmann-Sommergruber
Dec 5, 2008·Journal of Agricultural and Food Chemistry·Ivan López-ExpósitoRosina López-Fandiño
May 6, 2009·Molecular Nutrition & Food Research·Giuseppina MandalariE N Clare Mills

❮ Previous
Next ❯

Citations

Jan 1, 2011·Clinical and Translational Allergy·Apostolos BossiosPhotini Papageorgiou
Jun 4, 2010·Molecular Nutrition & Food Research·Didier DupontAlan R Mackie
Jan 22, 2015·Critical Reviews in Food Science and Nutrition·Katrine Lindholm Bøgh, Charlotte Bernhard Madsen
Dec 30, 2015·Critical Reviews in Food Science and Nutrition·David C DallasJ Bruce German
Nov 2, 2014·Soft Matter·Teresa del Castillo-SantaellaJulia Maldonado-Valderrama
Sep 7, 2014·Advances in Colloid and Interface Science·Julia Maldonado-ValderramaMiguel A Cabrerizo-Vílchez
Dec 22, 2012·International Archives of Allergy and Immunology·Katrine Lindholm BøghCharlotte Bernhard Madsen
Nov 1, 2017·Scientific Reports·Hasinika K A H GamageIan T Paulsen
Jan 20, 2015·Food & Function·S BenedéR López-Fandiño
Sep 12, 2017·Molecular Nutrition & Food Research·Junai GanJ Bruce German
Oct 20, 2018·Journal of the Science of Food and Agriculture·Mengmeng TianWei Zhou
Dec 24, 2010·Bipolar Disorders·Emily G SeveranceRobert H Yolken
Oct 31, 2012·The British Journal of Nutrition·Christine A ButtsPaul J Moughan
Sep 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·Steve SchulzYuri Gleba
Mar 2, 2012·The British Journal of Nutrition·Karima BouzerzourDidier Dupont
Oct 14, 2011·Food & Function·Revital Cohen BenshitritUri Lesmes
Dec 3, 2010·Allergy·I Pali-Schöll, E Jensen-Jarolim
Jun 21, 2020·Nutrients·Natalie AhlbornLinda M Samuelsson
Sep 20, 2017·Molecular Nutrition & Food Research·Gabriel MazzucchelliKarin Hoffmann-Sommergruber
May 21, 2020·Journal of Food Science and Technology·Shuyu XiangRoger Ruan
Sep 13, 2017·Journal of the Science of Food and Agriculture·Beatriz MirallesLourdes Amigo
Jun 11, 2019·Journal of the Science of Food and Agriculture·Yuxue SunMingruo Guo
Sep 27, 2014·Food & Function·Simon M LovedayGeoffrey B Jameson
Jan 18, 2020·Food Science of Animal Resources·Seonmin LeeSamooel Jung

❮ Previous
Next ❯

Related Concepts

Related Feeds