Comparative sequence analysis of envelope protein genes of Indian buffalopox virus isolates

Archives of Virology
Raj K SinghM P Yadav

Abstract

Buffalopox virus (BPXV) is considered to be a close variant of vaccinia virus (VACV), the prototype member of the genus Orthopoxvirus. In the present study, we have analyzed the sequences of H3L, A27L, and D8L gene-homologues of VACV in BPXV to elucidate its genetic relationship to VACV and other orthopoxviruses (OPVs). Products of these genes have been shown to be important in attachment of VACV to host cell surface receptors during viral entry. Additionally, the A27L gene is also responsible for cell fusion during infection, while the H3L gene is required for synthesis of the highly immunogenic major envelope protein p35. Full-length nucleotide sequences of H3L, A27L, and D8L genes of three BPXV isolates were determined by PCR amplification, cloning, and sequencing. The nucleotide (nt) sequence and the deduced amino acid (aa) sequences were compared with published sequences from other members of the genus Orthopoxvirus. Comparative sequence analysis of all the three genes revealed high sequence identity of BPXV isolates with VACV (close to 99% sequence identity) at both the nt and aa level. Phylogenetic analysis based on the deduced aa sequences of the H3L, A27L, and D8L genes also showed that BPXVs are more closely related t...Continue Reading

References

May 1, 1977·Tropical Animal Health and Production·S M Lal, I P Singh
Feb 1, 1981·Journal of Virological Methods·J EspositoJ Obijeski
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 16, 1999·Veterinary Radiology & Ultrasound : the Official Journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association·M KramerC Sheppard
Oct 1, 1958·The Biochemical Journal·R L SYNGE, J C WOOD
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Jan 18, 2005·Revista Do Instituto De Medicina Tropical De São Paulo·Teresa Keico Nagasse-SugaharaLuis Florêncio Salles-Gomes

❮ Previous
Next ❯

Citations

Jun 5, 2013·Indian Journal of Virology : an Official Organ of Indian Virological Society·R K SinghM Hosamani
Dec 18, 2013·PLoS Pathogens·Sergei N Shchelkunov
Jan 25, 2008·Emerging Infectious Diseases·Giliane S TrindadeInger K Damon
May 17, 2011·Veterinary Microbiology·B C BeraR K Singh
Oct 16, 2009·Veterinary Microbiology·Sandra EssbauerHermann Meyer
Jul 10, 2013·International Journal of Dermatology·Tarang GoyalRaj Kumar Singh
Aug 19, 2007·Animal Health Research Reviews·R K SinghM P Yadav
Aug 23, 2020·Pathogens·Kamal H EltomClaus-Peter Czerny

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.