PMID: 9650064Jul 3, 1998Paper

Comparative sequence analysis of the intergenic spacer region of cyathostome species

International Journal for Parasitology
J N KayeJ B McKeand

Abstract

The ribosomal DNA intergenic spacer was amplified by the polymerase chain reaction from 16 cyathostome species using primers derived from conserved regions within the flanking 18S and 26S rRNA genes. This generated a 1.5-2.5 kb fragment which was sequenced from five species. The areas covering the 26S and 18S rRNA genes were more than 99% similar among the five species. Furthermore, in all species there existed a highly conserved region of approximately 380 bp at the 3' end of the intergenic spacer. Subsequently, two cyathostome-specific primers were designed to amplify a smaller, more variable region of the intergenic spacer. Eleven further species were amplified using these primers and analysis showed that sequence similarities varied from 40 to 97% between species. The sequence information obtained in this study is being used to develop a PCR-based assay for the differentiation of preparasitic stages of cyathostomes.

References

Nov 1, 1992·Molecular and Biochemical Parasitology·P GuevaraJ L Ramírez
Mar 14, 1992·The Veterinary Record·S LoveM H Hillyer
May 18, 1989·Nature·S Kwok, R Higuchi
Jul 1, 1984·Veterinary Parasitology·C R ReinemeyerR P Herd

❮ Previous
Next ❯

Citations

Dec 11, 2008·Parasitology Research·Donato Traversa, Domenico Otranto
Nov 26, 2010·Parasitology Research·Tetiana A KuzminaEugene T Lyons
Mar 25, 2000·International Journal for Parasitology·R B Gasser, S E Newton
Mar 10, 2001·International Journal for Parasitology·J E HodgkinsonJ B Matthews
Oct 7, 2003·International Journal for Parasitology·J E HodgkinsonJ B Matthews
Oct 2, 2003·Veterinary Parasitology·M R ChapmanT R Klei
Sep 15, 1999·Veterinary Parasitology·S LoveD Mellor
Sep 21, 2010·Veterinary Parasitology·D C K van DoornH W Ploeger
Jul 14, 2017·Frontiers in Cellular and Infection Microbiology·Christina M BredtmannGeorg von Samson-Himmelstjerna
May 20, 2011·Equine Veterinary Journal·J B Matthews

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.