Comparative sporicidal effects of liquid chemical agents.

Applied and Environmental Microbiology
J L Sagripanti, A Bonifacino

Abstract

We compared the effectiveness of glutaraldehyde, formaldehyde, hydrogen peroxide, peracetic acid, cupric ascorbate (plus a sublethal amount of hydrogen peroxide), sodium hypochlorite, and phenol to inactivate Bacillus subtilis spores under various conditions. Each chemical agent was distinctly affected by pH, storage time after activation, dilution, and temperature. Only three of the preparations (hypochlorite, peracetic acid, and cupric ascorbate) studied here inactivated more than 99.9% of the spore load after a 30-min incubation at 20 degrees C at concentrations generally used to decontaminate medical devices. Under similar conditions, glutaraldehyde inactivated approximately 90%, and hydrogen peroxide, formaldehyde, and phenol produced little killing of spores in suspension. By kinetic analysis at different temperatures, we calculated the rate of spore inactivation (k) and the activation energy of spore killing (delta E) for each chemical agent. Rates of spore inactivation had a similar delta E value of approximately 20 kcal/mol (ca.83.68 kJ/mol) for every substance tested. The variation among k values allowed a quantitative comparison of liquid germicidal agents.

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Citations

Jul 9, 1999·Biotechnology and Bioengineering·K E LeJeuneA J Russell
Feb 22, 2003·Surgical Infections·J L Sagripanti, A Bonifacino
Aug 28, 2007·Applied and Environmental Microbiology·J HilgrenB Cords
Jul 17, 2008·Photochemistry and Photobiology·Thomas P Coohill, Jose-Luis Sagripanti
Aug 17, 2010·Applied and Environmental Microbiology·Ellen Raber, Alison Burklund
Aug 23, 2011·Applied and Environmental Microbiology·Jose-Luis SagripantiHans-Jürgen Marschall
Feb 23, 2012·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Chris G CampbellEllen Raber
Jun 22, 2012·Journal of Applied Microbiology·H Martin, P Maris
Mar 28, 2013·Journal of Industrial Microbiology & Biotechnology·Anne Cornish FrazerVishvesh K Bhupathiraju
Oct 17, 2013·Journal of Biomaterials Applications·Michael S ReichOzan Akkus
Aug 16, 2006·Journal of Applied Microbiology·P ScheldemanM Heyndrickx
Feb 27, 2008·Journal of Applied Microbiology·M CarreraJ-L Sagripanti
Dec 6, 2008·Letters in Applied Microbiology·J V RogersA K Judd
Mar 9, 2010·Applied and Environmental Microbiology·S StöckelJ Popp
Mar 12, 2013·Applied Microbiology and Biotechnology·Navdeep GroverJonathan S Dordick
Apr 10, 2008·Letters in Applied Microbiology·S M Retta, J-L Sagripanti
Jun 13, 2003·Microbiology and Immunology·Nori KidaFumiaki Taguchi
Sep 19, 2006·Applied and Environmental Microbiology·Timothy J J Inglis, Jose-Luis Sagripanti
Nov 4, 2017·Current Microbiology·Xuesong JiangHai-Quan Mao
Nov 5, 2005·Applied and Environmental Microbiology·Tatiana N Demidova, Michael R Hamblin
Mar 1, 2008·Microbial Biotechnology·Amalia RocaJuan L Ramos
Nov 29, 2012·Applied Microbiology and Biotechnology·Navdeep GroverJonathan S Dordick
Apr 12, 2013·Journal of Food Protection·Kingsley K AmoakoElizabeth Rohonczy
Oct 9, 2019·Journal of Exposure Science & Environmental Epidemiology·Sarah Ben MaamarErica M Hartmann
Oct 13, 2020·Food Science and Biotechnology·Won-Il Cho, Myong-Soo Chung
Nov 1, 2020·Applied and Environmental Microbiology·Takashi YokoyamaKatsuhisa Kitano
Jun 19, 2019·Emerging Infectious Diseases·Lahari SaikiaReeta Bora
Nov 16, 2017·Journal of Forensic Sciences·James M RobertsonRobert Bever

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