PMID: 698286Sep 4, 1978Paper

Comparative structural analysis of myosin after limited tryptic hydrolysis by use of two-dimensional gel electrophoresis

Biochimie
A Brevet, R G Whalen

Abstract

Polypeptides obtained by limited tryptic digestion of several myosins have been analyzed by a two-dimensional electrophoresis technique. The different maps thus obtained exhibit some common and distinct features specific of the myosins studied. Myosins from rabbit, fast and slow muscle and cardiac tissue, as well as beef cardiac myosin, have been compared. The polypeptides obtained vary in molecular weight from 120 000 60 15 000. The light chains LC1 and LC2, have disappeared. A peptide which comigrates with fast type LC3 is however found. The fast myosin hydrolyzate is very different from that obtained by the hydrolysis of slow and cardiac myosin. Numerous peptides are common to cardiac and slow myosins. However a few peptides are specific of the two myosin types. In the hydrolyzate from fetal calf myosin, some of the typical slow, fast and cardiac peptides can be found. However several apparently unique fetal peptides are also present. The comparison of the fetal calf tissue myosin hydrolyzate and that of [35S] methionine labeled myosin from myotubes in cultures shows qualitatively a very great homology. Thus the protein synthesized by cultured cells seems to be very similar to or the same as that of the embryonic tissue.

References

Nov 25, 1975·Journal of Molecular Biology·K Burridge, D Bray
Apr 25, 1975·Journal of Molecular Biology·C P Emerson, S K Beckner
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R G WhalenF Gros
May 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·S SarkarJ Gergely
Jun 8, 1973·Biochemical and Biophysical Research Communications·R CardinaudF Pelletier
Jan 18, 1974·Nature·A G WeedsA J Buller
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Feb 1, 1953·Archives of Biochemistry and Biophysics·A G SZENT-GYORGYI

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Citations

Jan 1, 1980·Histochemistry·R BilleterE Jenny
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R G WhalenF Gros
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H T NguyenB Nadal-Ginard
May 15, 1981·European Journal of Biochemistry·R BilleterE Jenny
Jun 29, 1984·Journal of Chromatography·I Syrový
Dec 14, 1979·Biochimica Et Biophysica Acta·L D LiberaS Schiaffino
Jun 15, 1983·Biochemical and Biophysical Research Communications·P A MerrifieldI R Konigsberg
Oct 31, 1980·Biochemical and Biophysical Research Communications·L Dalla LiberaS Schiaffino
Oct 1, 1985·Muscle & Nerve·D Pette, G Vrbová
Jan 1, 1982·Critical Reviews in Clinical Laboratory Sciences·C S Giometti

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